Anti-Phospholamban (phospho S16 + T17)抗体(ab62170)
Key features and details
- Rabbit polyclonal to Phospholamban (phospho S16 + T17)
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Phospholamban (phospho S16 + T17)抗体
参阅全部 Phospholamban 一抗 -
描述
兔多克隆抗体to Phospholamban (phospho S16 + T17) -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human Phospholamban (phospho S16 + T17).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62170于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
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| 说明 |
|---|
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ICC/IF
1/500 - 1/1000. |
靶标
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功能
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
组织特异性
Heart muscle (at protein level). -
疾病相关
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
序列相似性
Belongs to the phospholamban family. -
翻译后修饰
Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
细胞定位
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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数据库链接
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- SwissProt: P61014 Mouse
- SwissProt: P61016 Rat
- Unigene: 34145 Mouse
- Unigene: 9740 Rat
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别名
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Phospholamban (phospho S16 + T17) antibody (ab62170)Immunofluorescence analysis of HuvEc cells, using Phospholamban (phospho S16 + T17) antibody (ab62170) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab62170 被引用在 5 文献中.
- Gonçalves LS et al. Histidine dipeptides are key regulators of excitation-contraction coupling in cardiac muscle: Evidence from a novel CARNS1 knockout rat model. Redox Biol 44:102016 (2021). PubMed: 34038814
- Qin R et al. Exercise training reduces ventricular arrhythmias through restoring calcium handling and sympathetic tone in myocardial infarction mice. Physiol Rep 7:e13972 (2019). PubMed: 30806037
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Kim GE et al. LKB1 deletion causes early changes in atrial channel expression and electrophysiology prior to atrial fibrillation. Cardiovasc Res 108:197-208 (2015). PubMed: 26378152
- Huang Y et al. Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation. PLoS One 8:e82552 (2013). WB ; Rat . PubMed: 24386101