Anti-Phospho - (Ser/Thr) Phe抗体(ab17464)
Key features and details
- Rabbit polyclonal to Phospho - (Ser/Thr) Phe
- Suitable for: ELISA, IP, WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Phospho - (Ser/Thr) Phe抗体
参阅全部 Phospho - (Ser/Thr) Phe 一抗 -
描述
兔多克隆抗体to Phospho - (Ser/Thr) Phe -
宿主
Rabbit -
经测试应用
适用于: ELISA, IP, WB, ICC/IFmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
-
常规说明
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified by Protein A and peptide affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17464于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
1/500.
|
|
| IP |
1/100.
|
|
| WB | (5) |
1/1000.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
| ICC/IF | (1) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
ELISA
1/500. |
|
IP
1/100. |
|
WB
1/1000. Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20. |
|
ICC/IF
Use at an assay dependent concentration. |
图片
-
Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
-
Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
Lane 1 : Human THP-1 Whole Cell Extract
Lane 2 : Mouse Total Lung Extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Alkaline phosphatase conjugated goat anti-rabbit antibody
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 5 minutes
-
Western blot - Anti-Phospho - (Ser/Thr) Phe antibody (ab17464)All lanes : Anti-Phospho - (Ser/Thr) Phe antibody (ab17464) at 1/1000 dilution
All lanes : calyculin A treated A431 cells
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (106)
ab17464 被引用在 106 文献中.
- Hu LQ et al. Spatiotemporal formation of the large vacuole regulated by the BIN2-VLG module is required for female gametophyte development in Arabidopsis. Plant Cell 35:1241-1258 (2023). PubMed: 36648110
- Ao Y et al. Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence. Cells 12:N/A (2023). PubMed: 36831305
- Yu W et al. STK33 Phosphorylates Fibrous Sheath Protein AKAP3/4 to Regulate Sperm Flagella Assembly in Spermiogenesis. Mol Cell Proteomics 22:100564 (2023). PubMed: 37146716
- Zhao M et al. The cysteine-rich receptor-like kinase CRK4 contributes to the different drought stress response between Columbia and Landsberg erecta. Plant Cell Environ 46:3258-3272 (2023). PubMed: 37427814
- McDonald NA et al. SAD-1 kinase controls presynaptic phase separation by relieving SYD-2/Liprin-α autoinhibition. PLoS Biol 21:e3002421 (2023). PubMed: 38048304