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Anti-Phospho - (Ser/Thr)抗体(ab117253)

Reviews (1)Q&A (1)References (20)

Key features and details

  • Rabbit polyclonal to Phospho - (Ser/Thr)
  • Suitable for: ELISA, Dot blot
  • Reacts with: Species independent
  • Isotype: IgG

概述

  • 产品名称

    Anti-Phospho - (Ser/Thr)抗体

  • 描述

    兔多克隆抗体to Phospho - (Ser/Thr)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ELISA, Dot blotmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Phosphoserine/threonine conjugated with R-PE.

  • 常规说明

    Buffers and proteins which contain phosphate should be avoided with this antibody. Certain proteins known to contain phosphorylated serine and threonine may not be detected by this antibody due to steric hindrance.

    The immunogen used is Phosphoserine and phosphothreonine conjugated with R-PE.

     

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab117253于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ELISA
1/10000 - 1/50000.
Dot blot
Use at an assay dependent dilution.
说明
ELISA
1/10000 - 1/50000.
Dot blot
Use at an assay dependent dilution.

靶标

  • 相关性

    A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity.

  • 别名

    • Phosphoserine antibody
    • Phosphothreonine antibody

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (20)

发表研究结果有使用 ab117253？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab117253 被引用在 20 文献中.

  • Tang C  et al. Hippo signaling activates hedgehog signaling by Taz-driven Gli3 processing. Cell Regen 12:3 (2023). PubMed: 36720733
  • Luo Z  et al. Loss of phosphatase CTDNEP1 potentiates aggressive medulloblastoma by triggering MYC amplification and genomic instability. Nat Commun 14:762 (2023). PubMed: 36765089
  • Hu K  et al. Glutamate dehydrogenase1 supports HIF-1α stability to promote colorectal tumorigenesis under hypoxia. EMBO J 42:e112675 (2023). PubMed: 37092319
  • Zhao T  et al. NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity. EMBO J 42:e113258 (2023). PubMed: 37409632
  • Deng Q  et al. Smooth muscle liver kinase B1 inhibits foam cell formation and atherosclerosis via direct phosphorylation and activation of SIRT6. Cell Death Dis 14:542 (2023). PubMed: 37607939
View all Publications for this product

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Phosphoserine/threonine antibody

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Purified protein (recombinant protein)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
2 µg
Treatment
Kinase domain of Obscurin
Specification
recombinant protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Li Wang

Verified customer

提交于 Mar 25 2016

Question

Thanks for your information. It is glad to hear that it is Pan-specific phosphoserine antibody. I am going to use it to detect the phosphorylation of purified recombinant dynein intermediate chain of  mouse origin by a Ser/Thr kinase. Although I am not sure it's serine phosphorylation, it has higher possibility than Thr phosphorylation. Therefore, I am wondering if you could help me check if Abcam has  pan-specific phospho-Ser/Thr  antibody. Otherwise, I am going to get  this Pan-specific phosphoserine antibody and pan-specific phosphothreonine antibody(if there is any). if our research is qualified for discount, please let me know. Thanks

Read More
Answer

We do offer two products which may meet your needs, ab17464 and ab117253.

While not truly Pan phospho Ser/ThrAb14764 detects phosphoserine or phosphothreonine in the context of tyrosine, tryptophan or phenylalanine at the -1 position or phenylalanine at the +1 position. This product has been tested and guaranteed to work inICC/IF,ELISA,IP,and WB and has been referenced in seven publications to our knowledge. The product page is:

https://www.abcam.com/Phosphoserine-threonine-antibody-ab17464.html

Ab117253detects free and conjugated Phosphoserine/threonine as well as their modified cellular proteins. It does not cross-react with nonphosphorylated tyrosine, serine, threonine, AMP, or ATP. It has been tested and guaranteed to work inELISA,Dot Blot on human tissues. Again, I would like you to be aware that for each antibody of this type certain proteins known to contain phosphorylated serine and threonine may not be detected due to steric hindrance.The product page for this is:

https://www.abcam.com/Phosphoserine-threonine-antibody-ab117253.html

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

To determine if one of these products would qualify for participation in our 100% Abreview testing discount program could you tell me more about the application and species you would be testing the product on?

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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