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重组RabMAb

重组Anti-PHLPP2抗体[SP220] - BSA and Azide free (ab272010)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
  • Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP220] to PHLPP2 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-PHLPP2抗体[SP220] - BSA and Azide free
    参阅全部 PHLPP2 一抗

  • 描述

    兔单克隆抗体[SP220] to PHLPP2 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human prostate, and prostate carcinoma tissue;WB: HEK293T cell lysate.

  • 常规说明

    ab272010 is the carrier-free version of ab227673.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272010于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval by boiling tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary antibody incubation for 10 minutes at room temperature.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 147 kDa.

Primary antibody incubation for 1 hour at room temperature.

 
说明
IHC-P
Use at an assay dependent concentration.

Perform heat mediated antigen retrieval by boiling tissue section in citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary antibody incubation for 10 minutes at room temperature.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 147 kDa.

Primary antibody incubation for 1 hour at room temperature.

 

靶标

  • 功能

    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.

  • 组织特异性

    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).

  • 序列相似性

    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.

  • 细胞定位

    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • KIAA0931 antibody
      • PH domain and leucine rich repeat protein phosphatase 2 antibody
      • PH domain leucine rich repeat protein phosphatase like antibody
      • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
      • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
      • PHLP2_HUMAN antibody
      • PHLPP like antibody
      • PHLPP-like antibody
      • Phlpp2 antibody
      • PHLPPL antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostate carcinoma tissue sections labeling PHLPP2 with ab227673 at 1/100 dilution (1.65 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227673).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)

      Formalin-fixed, paraffin-embedded human prostate tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227673).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)

      Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PHLPP2 using ab227673 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227673).
    • Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)
      Anti-PHLPP2 antibody [SP220] - BSA and Azide free (ab272010)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab272010？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272010 尚未被引用在任何文献中。

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