Anti-PHLPP2抗体(ab71973)
Key features and details
- Rabbit polyclonal to PHLPP2
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PHLPP2抗体
参阅全部 PHLPP2 一抗 -
描述
兔多克隆抗体to PHLPP2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Guinea pig, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide corresponding to a region between residues 1275 and the C-terminus (residue 1323) of human PHLPP2 (NP_055835.2)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab71973于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa).
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| IP |
Use at 1-4 µg/mg of lysate.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa). |
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IP
Use at 1-4 µg/mg of lysate. |
靶标
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功能
Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. -
组织特异性
In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). -
序列相似性
Contains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
细胞定位
Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 23035 Human
- Entrez Gene: 244650 Mouse
- Omim: 611066 Human
- SwissProt: Q6ZVD8 Human
- SwissProt: Q8BXA7 Mouse
- Unigene: 709458 Human
- Unigene: 23494 Mouse
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别名
- KIAA0931 antibody
- PH domain and leucine rich repeat protein phosphatase 2 antibody
- PH domain leucine rich repeat protein phosphatase like antibody
see all
图片
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Western blot - Anti-PHLPP2 antibody (ab71973)All lanes : Anti-PHLPP2 antibody (ab71973) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 147 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-PHLPP2 antibody (ab71973)Detection of PHLPP2 by Western Blot of Immunoprecipitate.
ab71973 at 1µg/ml staining PHLPP2 in HeLa whole cell lysate immunoprecipitated using ab71973 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab71973 被引用在 18 文献中.
- Shi X et al. Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer. Gastroenterology 162:2004-2017.e2 (2022). PubMed: 35176309
- Tantai J et al. TRIM46 activates AKT/HK2 signaling by modifying PHLPP2 ubiquitylation to promote glycolysis and chemoresistance of lung cancer cells. Cell Death Dis 13:285 (2022). PubMed: 35354796
- Huang J et al. ZDHHC22-mediated mTOR palmitoylation restrains breast cancer growth and endocrine therapy resistance. Int J Biol Sci 18:2833-2850 (2022). PubMed: 35541896
- Nayak RC et al. Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis. Nat Commun 13:3056 (2022). PubMed: 35650206
- Li Y et al. Novel mechanisms underlying inhibition of inflammation-induced angiogenesis by dexamethasone and gentamicin via PI3K/AKT/NF-κB/VEGF pathways in acute radiation proctitis. Sci Rep 12:14116 (2022). PubMed: 35982137