Anti-PHLDA3抗体(ab22822)
Key features and details
- Goat polyclonal to PHLDA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PHLDA3抗体
参阅全部 PHLDA3 一抗 -
描述
山羊多克隆抗体to PHLDA3 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide:
RARQSLGTGTLVS
, corresponding to C terminal amino acids 115-127 of Human PHLDA3. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab22822 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab22822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
A 1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa). A 1 hour primary incubation is recommended for this product. |
靶标
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功能
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. -
组织特异性
Widely expressed with lowest expression in liver and spleen. -
序列相似性
Belongs to the PHLDA3 family.
Contains 1 PH domain. -
结构域
The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23612 Human
- Entrez Gene: 27280 Mouse
- Entrez Gene: 363989 Rat
- Omim: 607054 Human
- SwissProt: Q9Y5J5 Human
- SwissProt: Q9WV95 Mouse
- SwissProt: Q5PQT7 Rat
- Unigene: 268557 Human
see all -
别名
- PHLA3_HUMAN antibody
- PHLDA 3 antibody
- phlda3 antibody
see all
图片
数据表及文件
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Datasheet download
文献 (4)
ab22822 被引用在 4 文献中.
- Bensellam M et al. Phlda3 regulates beta cell survival during stress. Sci Rep 9:12827 (2019). PubMed: 31492921
- Leszczynska KB et al. Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT. J Clin Invest 125:2385-98 (2015). PubMed: 25961455
- Ohki R et al. PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors. Proc Natl Acad Sci U S A 111:E2404-13 (2014). PubMed: 24912192
- Kawase T et al. PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt. Cell 136:535-50 (2009). WB . PubMed: 19203586