AB22822

Anti-PHLDA3抗体

Name: PHLDA3抗体 (ab22822)| Abcam中文官网
Brand: Abcam Limited
SKU: AB22822
Price: 3069 CNY
Availability: InStock
Rating: 4 (1 reviews)

Anti-PHLDA3 antibody

(1 Review)

(5 Publications)

Goat Polyclonal PHLDA3 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PHLDA3 aa 100 to C-terminus.

查看别名

TIH1, PHLDA3, Pleckstrin homology-like domain family A member 3, TDAG51/Ipl homolog 1

1 Images

Unknown

Western blot - Anti-PHLDA3 antibody (AB22822)

Lanes 1 - 2:

Western blot - Anti-PHLDA3 antibody (ab22822) at 1 µg/mL

Lane 3:

Anti-myc tag antibody at 1/1000 dilution

Lanes 1 and 3:

HEK293 lysate overexpressing Human PHLDA3 in RIPA buffer at 10 µg

Lane 2:

Mock-transfected HEK293

Predicted band size: 14 kDa

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human PHLDA3 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5J5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab22822 was purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PHLDA3 also known as PHLDA family member 3 is a small protein with a molecular mass of about 15 kDa. It functions mechanically as an antagonist of the AKT signaling pathway. PHLDA3 can inhibit AKT by binding to its pleckstrin homology (PH) domain preventing AKT's interaction with other molecules. This protein is expressed in various tissues throughout the body including the brain lungs and liver indicating its role in diverse cellular functions.

Biological function summary

The protein acts as a tumor suppressor through its ability to regulate cell growth and apoptosis. PHLDA3 does this by modulating the AKT pathway inhibiting cell survival and promoting apoptosis. It is not part of a larger complex but interacts directly with other proteins to execute its function. The influence of PHLDA3 on cell cycle processes suggests it plays roles in maintaining normal cellular functions and preventing uncontrolled proliferation.

Pathways

Several studies have shown PHLDA3 integrates into the p53 signaling pathway a critical pathway for cellular stress responses and tumor suppression. It interacts with the AKT pathway which connects it to proteins like PI3K and PTEN influencing cellular growth and apoptosis. PHLDA3 effectively bridges interaction between the p53 pathway and AKT pathway which balances cell fate decisions under stress conditions.

PHLDA3 exhibits a significant impact on cancer development and progression. Loss of PHLDA3 expression has been observed in various tumors suggesting its role in cancer development. Additionally it relates to diabetes where altered AKT signaling affects insulin response. In cancer PHLDA3 connects to the tumor suppressor protein p53 influencing its regulatory functions while in diabetes its relation to AKT pathway points to disturbances in glucose homeostasis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.

See full target information PHLDA3

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 21:613 PubMed33936270

2021

PHLDA3 inhibition attenuates endoplasmic reticulum stress-induced apoptosis in myocardial hypoxia/reoxygenation injury by activating the PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Ying Chen,Fen Ai,Yun-Qian Li,Kun Zhang,Wei-Tong Zhang

Scientific reports 9:12827 PubMed31492921

2019

Phlda3 regulates beta cell survival during stress.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Bensellam,Jeng Yie Chan,Kailun Lee,Mugdha V Joglekar,Anandwardhan A Hardikar,Thomas Loudovaris,Helen E Thomas,Jean-Christophe Jonas,D Ross Laybutt

The Journal of clinical investigation 125:2385-98 PubMed25961455

2015

Hypoxia-induced p53 modulates both apoptosis and radiosensitivity via AKT.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna B Leszczynska,Iosifina P Foskolou,Aswin G Abraham,Selvakumar Anbalagan,Céline Tellier,Syed Haider,Paul N Span,Eric E O'Neill,Francesca M Buffa,Ester M Hammond

Proceedings of the National Academy of Sciences of 111:E2404-13 PubMed24912192

2014

PHLDA3 is a novel tumor suppressor of pancreatic neuroendocrine tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Rieko Ohki,Kozue Saito,Yu Chen,Tatsuya Kawase,Nobuyoshi Hiraoka,Raira Saigawa,Maiko Minegishi,Yukie Aita,Goichi Yanai,Hiroko Shimizu,Shinichi Yachida,Naoaki Sakata,Ryuichiro Doi,Tomoo Kosuge,Kazuaki Shimada,Benjamin Tycko,Toshihiko Tsukada,Yae Kanai,Shoichiro Sumi,Hideo Namiki,Yoichi Taya,Tatsuhiro Shibata,Hitoshi Nakagama

Cell 136:535-50 PubMed19203586

2009

PH domain-only protein PHLDA3 is a p53-regulated repressor of Akt.

Applications

Species

Unspecified reactive species

Tatsuya Kawase,Rieko Ohki,Tatsuhiro Shibata,Shuichi Tsutsumi,Naoko Kamimura,Johji Inazawa,Tsutomu Ohta,Hitoshi Ichikawa,Hiroyuki Aburatani,Fumio Tashiro,Yoichi Taya

View all publications

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Anti-PHLDA3抗体

Anti-PHLDA3 antibody

Western blot - Anti-PHLDA3 antibody (AB22822)