Anti-PHGDH/Malate dehydrogenase抗体[4A3-1D6] (ab57030)
![Western blot - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)](https://www.abcam.cn/ps/products/57/ab57030/Images/ab57030_1.jpg "Western blot - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)")
Key features and details
- Mouse monoclonal [4A3-1D6] to PHGDH/Malate dehydrogenase
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-PHGDH/Malate dehydrogenase抗体[4A3-1D6]
参阅全部 PHGDH/Malate dehydrogenase 一抗 -
描述
小鼠单克隆抗体[4A3-1D6] to PHGDH/Malate dehydrogenase -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human PHGDH/Malate dehydrogenase aa 1-550.
Database link: O43175 -
常规说明
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4 -
Concentration information loading... -
纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
4A3-1D6 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab57030于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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| ICC/IF |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
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ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IP
Use at an assay dependent concentration. |
靶标
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通路
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3. -
疾病相关
Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures. -
序列相似性
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. - Information by UniProt
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数据库链接
- Entrez Gene: 26227 Human
- Omim: 606879 Human
- SwissProt: O43175 Human
- Unigene: 487296 Human
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别名
- 3 PGDH antibody
- 3-PGDH antibody
- 3-phosphoglycerate dehydrogenase antibody
see all
图片
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Western blot - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)
PHGDH/Malate dehydrogenase antibody (ab57030) at 1μg/ml + Jurkat cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)](https://www.abcam.cn/ps/products/57/ab57030/Images/ab57030_2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)PHGDH/Malate dehydrogenase antibody (ab57030) used in immunofluorescence at 1-5μg/ml on HeLa cells.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)](https://www.abcam.cn/ps/products/57/ab57030/Images/ab57030_3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)PHGDH/Malate dehydrogenase antibody (ab57030) used in immunohistochemistry at 5μg/ml on formalin fixed and paraffin embedded human lymph node.
This image was generated using the ascites version of the product.
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![Immunoprecipitation - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)](https://www.abcam.cn/ps/products/57/ab57030/Images/PHGDH-Primary-antibodies-ab57030-3.jpg)
Immunoprecipitation - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)PHGDH/Malate dehydrogenase was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Mouse monoclonal to PHGDH/Malate dehydrogenase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57030.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 57kDa; PHGDH/Malate dehydrogenase.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)](https://www.abcam.cn/ps/products/57/ab57030/Images/PHGDH-Primary-antibodies-ab57030-2.jpg)
Flow Cytometry - Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6] (ab57030)Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (13)
ab57030 被引用在 13 文献中.
- Wang K et al. PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma. Nat Commun 14:1011 (2023). PubMed: 36823188
- Naka T et al. Identification of Novel Diagnostic Markers for Malignant Pleural Mesothelioma Using a Reverse Translational Approach Based on a Rare Synchronous Tumor. Diagnostics (Basel) 12:N/A (2022). PubMed: 35204409
- Yao S et al. One carbon metabolism in human lung cancer. Transl Lung Cancer Res 10:2523-2538 (2021). PubMed: 34295659
- You MH et al. Death-Associated Protein Kinase 1 Inhibits Progression of Thyroid Cancer by Regulating Stem Cell Markers. Cells 10:N/A (2021). PubMed: 34831219
- Piga I et al. In situ Metabolic Profiling of Ovarian Cancer Tumor Xenografts: A Digital Pathology Approach. Front Oncol 10:1277 (2020). PubMed: 32974128
