Anti-PHF21B抗体[OTI3D1] (ab119249)
![Western blot - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-1.jpg "Western blot - Anti-PHF21B antibody [OTI3D1] (ab119249)")
Key features and details
- Mouse monoclonal [OTI3D1] to PHF21B
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-PHF21B抗体[OTI3D1] -
描述
小鼠单克隆抗体[OTI3D1] to PHF21B -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human PHF21B. Produced in HEK293T cells (NP_612424.1).
Database link: Q96EK2 -
阳性对照
- IHC-P: Human Breast, Kidney, Endometrium, Liver, Ovary Adenocarcinoma, Pancreas carcinoma, Lung carcinoma and Thyroid Carcinoma tissues. WB: HEK-293T cell lysate transfected with pCMV6-ENTRY PHF21B cDNA. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY PHF21B. Flow Cyt (Intra): HEK-293T cells transfected with pCMV6-ENTRY PHF21B overexpress plasmid.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI3D1 -
同种型
IgG2b -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab119249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/2000. Predicted molecular weight: 57 kDa.
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| IHC-P |
1/150.
|
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| ICC/IF |
1/100.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB
1/2000. Predicted molecular weight: 57 kDa. |
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IHC-P
1/150. |
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ICC/IF
1/100. |
靶标
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序列相似性
Contains 1 PHD-type zinc finger. - Information by UniProt
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数据库链接
- Entrez Gene: 112885 Human
- SwissProt: Q96EK2 Human
- Unigene: 254097 Human
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别名
- A730032D07Rik antibody
- AL049760.6 antibody
- BHC80L antibody
see all
图片
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Western blot - Anti-PHF21B antibody [OTI3D1] (ab119249)All lanes : Anti-PHF21B antibody [OTI3D1] (ab119249) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY
control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY
PHF21B cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 57 kDa -
![Flow Cytometry (Intracellular) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-2.jpg)
Flow Cytometry (Intracellular) - Anti-PHF21B antibody [OTI3D1] (ab119249)Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY PHF21B overexpress plasmid (Red) or empty vector control plasmid (Blue), stained using ab119249 at 1/100 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-3.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHF21B antibody [OTI3D1] (ab119249)COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY PHF21B, stained for PHF21B (green) using ab119249 at 1/100 dilution in ICC/IF.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human breast tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/ab119249-132168-anti-phf21b-antibody-oti3d1-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human endometrium tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-8.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human kidney tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-9.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human liver tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-10.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human lung carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-11.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human ovary adenocarcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-12.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human pancreas carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)](https://www.abcam.cn/ps/products/119/ab119249/Images/PHF21B-Primary-antibodies-ab119249-13.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHF21B antibody [OTI3D1] (ab119249)Paraffin-embedded human breast thyroid carcinoma tissue stained for PHF21B using ab119249 at 1/150 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119249 尚未被引用在任何文献中。