重组Anti-PHD1/prolyl hydroxylase抗体[EPR2745] (ab108980)
![Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)](https://www.abcam.cn/ps/products/108/ab108980/Images/ab108980-121490-anti-phd1-prolyl-hydroxylase-antibody-epr2745-western-blot.jpg "Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PHD1/prolyl hydroxylase抗体[EPR2745]
参阅全部 PHD1/prolyl hydroxylase 一抗 -
描述
兔单克隆抗体[EPR2745] to PHD1/prolyl hydroxylase -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- A549,HeLa, and MCF-7 cell lysates Human ovarian adenocarcinoma tissue Human testis tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2745 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Alexa Fluor® 488 Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab202890)
- Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free (ab247731)
- Alexa Fluor® 568 Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab312557)
- Alexa Fluor® 750 Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab320921)
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108980于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval |
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval |
|
ICC/IF
1/100 - 1/250. |
靶标
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功能
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity. -
组织特异性
Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. -
序列相似性
Contains 1 Fe2OG dioxygenase domain. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 112398 Human
- Entrez Gene: 112406 Mouse
- Entrez Gene: 308457 Rat
- Omim: 606424 Human
- SwissProt: Q96KS0 Human
- SwissProt: Q91YE2 Mouse
- SwissProt: Q6AYU4 Rat
- Unigene: 515417 Human
see all -
别名
- DKFZp434E026 antibody
- EGL nine (C.elegans) homolog 2 antibody
- Egl nine homolog 2 (C. elegans) antibody
see all
图片
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Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 44 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)](https://www.abcam.cn/ps/products/108/ab108980/Images/ab108980-121489-anti-phd1-prolyl-hydroxylase-antibody-epr2745-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)](https://www.abcam.cn/ps/products/108/ab108980/Images/ab108980-121488-anti-phd1-prolyl-hydroxylase-antibody-epr2745-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)](https://www.abcam.cn/ps/products/108/ab108980/Images/ab108980-121487-anti-phd1-prolyl-hydroxylase-antibody-epr2745-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)Immunofluorescent staining of HeLa cells using ab108980 -
![Flow Cytometry (Intracellular) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)](https://www.abcam.cn/ps/products/108/ab108980/Images/ab108980-121486-anti-phd1-prolyl-hydroxylase-antibody-epr2745-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)](https://www.abcam.cn/ps/products/108/ab108980/Images/ab108980-6-benefits-of-recombinant-antibodies.png)
Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab108980 被引用在 19 文献中.
- Noonan ML et al. Osteocyte Egln1/Phd2 links oxygen sensing and biomineralization via FGF23. Bone Res 11:7 (2023). PubMed: 36650133
- Navarrete C et al. A cannabidiol aminoquinone derivative activates the PP2A/B55α/HIF pathway and shows protective effects in a murine model of traumatic brain injury. J Neuroinflammation 19:177 (2022). PubMed: 35810304
- Ito R et al. SM22α cell-specific HIF stabilization mitigates hyperoxia-induced neonatal lung injury. Am J Physiol Lung Cell Mol Physiol 323:L129-L141 (2022). PubMed: 35762602
- Silva-Luna K et al. Prolidase expression in knee osteoarthritis and healthy controls: Observational study. Medicine (Baltimore) 100:e27059 (2021). PubMed: 34477138
- Lin Q et al. Inhibiting NLRP3 inflammasome attenuates apoptosis in contrast-induced acute kidney injury through the upregulation of HIF1A and BNIP3-mediated mitophagy. Autophagy 17:2975-2990 (2021). PubMed: 33345685