AB108980

Anti-PHD1/prolyl hydroxylase 抗体 [EPR2745]

Name: PHD1/prolyl hydroxylase抗体[EPR2745] (ab108980)| Abcam中文官网
Brand: Abcam Limited
SKU: AB108980
Price: 2406 CNY
Availability: InStock
Rating: 2 (1 reviews)

Anti-PHD1/prolyl hydroxylase antibody [EPR2745]

RabMAb
Recombinant
了解详情

(1 Review)

(23 Publications)

Rabbit Recombinant Monoclonal PHD1/prolyl hydroxylase antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 23 publications.

查看别名

EIT6, EGLN2, Prolyl hydroxylase EGLN2, Egl nine homolog 2, Estrogen-induced tag 6, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 1, Prolyl hydroxylase domain-containing protein 1, EIT-6, HIF-PH1, HIF-prolyl hydroxylase 1, HPH-1, PHD1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

Immunofluorescent staining of HeLa cells using ab108980

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5000 events was performed.

Unknown

Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (AB108980)

All lanes:

Western blot - Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 44 kDa

false

不同偶联物与剂型 (9)

Carrier free

Anti-PHD1/prolyl hydroxylase antibody [EPR2745] - BSA and Azide free
578 PE

PE Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
660 APC

APC Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
HRP

HRP Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-PHD1/prolyl hydroxylase antibody [EPR2745]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2745

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, Flow Cyt (Intra), WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "Perform antigen retrieval Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Prolyl hydroxylase 1 (PHD1) also known as EGLN2 is an enzyme that hydroxylates specific proline residues within target proteins. This process of prolyl hydroxylation is critical for regulating protein stability. PHD1 shares structural similarities with other members of the 2-oxoglutarate-dependent dioxygenase family and includes a conserved iron-binding motif essential for its enzymatic activity. PHD1 has a molecular mass of approximately 46 kDa and is expressed in a variety of tissues with notable presence in skeletal muscle heart and the liver.

Biological function summary

PHD1 modulates the stability of the hypoxia-inducible factor (HIF) proteins which are key regulators of oxygen homeostasis in the cell. Under normoxic conditions PHD1 hydroxylates specific proline residues on HIF-alpha marking it for degradation via the ubiquitin-proteasome pathway. This enzyme does not function as part of a larger protein complex but it plays a pivotal role in determining the cellular response to oxygen levels. Additionally PHD1 expression affects the metabolic adaptation processes and energy expenditure in cells.

Pathways

PHD1 plays a role in the cellular response to hypoxia and is integral to the HIF signaling pathway. It interacts directly with HIF-alpha subunits mediating their degradation under normal oxygen conditions to ensure HIF activity remains inhibited. Additionally PHD1 is indirectly involved in modulating the angiogenesis pathway as it influences the availability of HIF-related transcription factors which promote transcription of vascular endothelial growth factor (VEGF) under low oxygen conditions. The interplay between PHD1 PHD2 and PHD3 ensures a fine-tuned regulation of the HIF pathway based on oxygen availability.

PHD1 has links to cancer progression and ischemic conditions. The enzyme’s activity is often altered in response to the aberrant hypoxic signaling found within tumors impacting cellular proliferation and survival. In ischemic conditions reduced PHD1 activity leads to stabilization of HIF proteins and adaptation responses aimed at tissue survival. Mutations or dysregulation in PHD1 expression have been observed in metabolic syndromes suggesting potential therapeutic targets. Through its control over hypoxia-related proteins PHD1 also interacts with von Hippel-Lindau (VHL) protein which is part of the E3 ubiquitin ligase complex important for HIF-alpha degradation.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519, PubMed : 16509823, PubMed : 17114296, PubMed : 23932902). Target proteins are preferentially recognized via a LXXLAP motif (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins (PubMed : 11595184, PubMed : 12039559, PubMed : 12181324, PubMed : 15925519, PubMed : 19339211). Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A (PubMed : 11595184, PubMed : 12039559, PubMed : 12181324, PubMed : 15925519). Also hydroxylates HIF2A (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). Has a preference for the CODD site for both HIF1A and HIF2A (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). Also regulates susceptibility to normoxic oxidative neuronal death (PubMed : 11595184, PubMed : 12039559, PubMed : 15925519). Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation (PubMed : 23932902). Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions (PubMed : 17114296). Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 (By similarity).

See full target information EGLN2

style

left-column

文献 (23)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in physiology 15:1488152 PubMed39882327

2025

Chronic fetal hypoxia and antenatal Vitamin C exposure differentially regulate molecular signalling in the lung of female lambs in early adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Erin V McGillick,Sandra Orgeig,Beth J Allison,Kirsty L Brain,Melanie R Bertossa,Stacey L Holman,Ashley S Meakin,Michael D Wiese,Youguo Niu,Nozomi Itani,Katie L Skeffington,Christian Beck,Kimberly J Botting-Lawford,Janna L Morrison,Dino A Giussani

Journal of translational medicine 22:248 PubMed38454480

2024

Inhibition of OGFOD1 by FG4592 confers neuroprotection by activating unfolded protein response and autophagy after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Xie,Yuan Zhang,Bin Li,Wen Xi,Yu Wang,Lu Li,Chenchen Liu,Ling Shen,Bing Han,Yan Kong,HongHong Yao,Zhijun Zhang

American journal of physiology. Lung cellular and molecular physiology 325:L741-L755 PubMed37847687

2023

Loss of prolyl hydroxylase 1 and 2 in SM22α-expressing cells prevents Hypoxia-Induced pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Barnes,Reiji Ito,Xibing Che,Cristina M Alvira,David N Cornfield

Bone research 11:7 PubMed36650133

2023

Osteocyte Egln1/Phd2 links oxygen sensing and biomineralization via FGF23.

Applications

Unspecified application

Species

Unspecified reactive species

Megan L Noonan,Pu Ni,Emmanuel Solis,Yamil G Marambio,Rafiou Agoro,Xiaona Chu,Yue Wang,Hongyu Gao,Xiaoling Xuei,Erica L Clinkenbeard,Guanglong Jiang,Sheng Liu,Steve Stegen,Geert Carmeliet,William R Thompson,Yunlong Liu,Jun Wan,Kenneth E White

Journal of neuroinflammation 19:177 PubMed35810304

2022

A cannabidiol aminoquinone derivative activates the PP2A/B55α/HIF pathway and shows protective effects in a murine model of traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Navarrete,Adela García-Martín,Alejandro Correa-Sáez,María E Prados,Francisco Fernández,Rafael Pineda,Massimiliano Mazzone,Marina Álvarez-Benito,Marco A Calzado,Eduardo Muñoz

American journal of physiology. Lung cellular and molecular physiology 323:L129-L141 PubMed35762602

2022

SM22α cell-specific HIF stabilization mitigates hyperoxia-induced neonatal lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Reiji Ito,Elizabeth A Barnes,Xibing Che,Cristina M Alvira,David N Cornfield

Medicine 100:e27059 PubMed34477138

2021

Prolidase expression in knee osteoarthritis and healthy controls: Observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Silva-Luna,Lucio Ventura-Ríos,Ambar López-Macay

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 18:1849-1861 PubMed34339019

2021

Betulinic Acid Hydroxamate is Neuroprotective and Induces Protein Phosphatase 2A-Dependent HIF-1α Stabilization and Post-transcriptional Dephosphorylation of Prolyl Hydrolase 2.

Applications

Unspecified application

Species

Unspecified reactive species

María E Prados,Alejandro Correa-Sáez,Juan D Unciti-Broceta,Martín Garrido-Rodríguez,Carla Jimenez-Jimenez,Massimiliano Mazzone,Alberto Minassi,Giovanni Appendino,Marco A Calzado,Eduardo Muñoz

Frontiers in cell and developmental biology 9:670913 PubMed34178995

2021

TMSB4 Overexpression Enhances the Potency of Marrow Mesenchymal Stromal Cells for Myocardial Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyuan Tang,Chengming Fan,Chukwuemeka Daniel Iroegbu,Wenwu Zhou,Zhigong Zhang,Ming Wu,Wangping Chen,Xiaoming Wu,Jun Peng,Zhihong Li,Jinfu Yang

Molecular therapy : the journal of the American Society of Gene Therapy 29:2134-2150 PubMed33601056

2021

WDR5 facilitates EMT and metastasis of CCA by increasing HIF-1α accumulation in Myc-dependent and independent pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Tianli Chen,Kangshuai Li,Zengli Liu,Jialiang Liu,Yue Wang,Rongqi Sun,Zhipeng Li,Bo Qiu,Xiaoming Zhang,Guangli Ren,Yunfei Xu,Zongli Zhang

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PHD1/prolyl hydroxylase 抗体 [EPR2745]