重组Anti-PHD finger蛋白6/PHF6抗体[EPR11997] (ab173304)
![Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)](https://www.abcam.cn/ps/products/173/ab173304/Images/ab173304-347007-anti-phd-finger-protein-6-antibody-epr11997-westernblot-knock-out-human.jpg "Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11997] to PHD finger protein 6/PHF6
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PHD finger蛋白6/PHF6抗体[EPR11997]
参阅全部 PHD finger protein 6/PHF6 一抗 -
描述
兔单克隆抗体[EPR11997] to PHD finger蛋白6/PHF6 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa, A431, HEK-293, K562, and 293T cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11997 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173304于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 41 kDa.
|
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| ICC/IF |
1/50 - 1/100.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 41 kDa. |
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ICC/IF
1/50 - 1/100. |
靶标
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功能
May play a role in transcriptional regulation. -
组织特异性
Ubiquitously expressed. -
疾病相关
Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears. -
序列相似性
Contains 2 PHD-type zinc fingers. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 84295 Human
- Entrez Gene: 70998 Mouse
- Entrez Gene: 100359714 Rat
- Omim: 300414 Human
- SwissProt: Q8IWS0 Human
- SwissProt: Q9D4J7 Mouse
- Unigene: 356501 Human
- Unigene: 26870 Mouse
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别名
- AC004383.6 antibody
- BFLS antibody
- BORJ antibody
see all
图片
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Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : PHF6 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under non-reducing conditions.
Predicted band size: 41 kDaLanes 1 - 4: Merged signal (red and green). Green - ab173304 observed at 41 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab173304 was shown to recognize PHD finger protein 6 in wild-type HEK-293 cells as signal was lost at the expected MW in PHF6 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and PHF6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab173304 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)](https://www.abcam.cn/ps/products/173/ab173304/Images/ab173304-196767-anti-phd-finger-protein-6-antibody-epr11997-western-blot.jpg)
Western blot - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)All lanes : Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 41 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)](https://www.abcam.cn/ps/products/173/ab173304/Images/ab173304-196765-anti-phd-finger-protein-6-antibody-epr11997-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)Immunofluorescent analysis of HeLa cells labeling PHD finger protein 6/PHF6 with ab173304 at 1/50 dilution.
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![Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)](https://www.abcam.cn/ps/products/173/ab173304/Images/ab173304-5-benefits-of-recombinant-antibodies.png)
Anti-PHD finger protein 6/PHF6 antibody [EPR11997] (ab173304)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab173304 被引用在 2 文献中.
- Yu Q et al. Long non-coding RNA LHX1-DT regulates cardiomyocyte differentiation through H2A.Z-mediated LHX1 transcriptional activation. iScience 26:108051 (2023). PubMed: 37942009
- Cai T et al. Notoginsenoside R1 induces DNA damage via PHF6 protein to inhibit cervical carcinoma cell proliferation. Mol Med Rep 23:N/A (2021). PubMed: 33537816