Anti-PGP9.5抗体- Neuronal Marker (ab15503)
Key features and details
- Rabbit polyclonal to PGP9.5 - Neuronal Marker
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PGP9.5抗体- Neuronal Marker
参阅全部 PGP9.5 一抗 -
描述
兔多克隆抗体to PGP9.5 - Neuronal Marker -
宿主
Rabbit -
特异性
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab108986.
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经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human PGP9.5.
Database link: P09936 -
阳性对照
- Pancreas and small intestine This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.
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常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15503于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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IHC-P | (3) |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Cool at RT for 20 minutes.
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说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Cool at RT for 20 minutes. |
靶标
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功能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
组织特异性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
疾病相关
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
序列相似性
Belongs to the peptidase C12 family. -
翻译后修饰
O-glycosylated. -
细胞定位
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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数据库链接
- Entrez Gene: 7345 Human
- Omim: 191342 Human
- SwissProt: P09936 Human
- Unigene: 518731 Human
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别名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
图片
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for PGP9.5 using ab15503 at 1/200 dilution in immunohistochemical analysis.
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ICC/IF image of ab15503 stained DU145. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15503 at 1 ug/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Anti-PGP9.5 antibody - Neuronal Marker (ab15503) at 1/25 dilution + Human Brain lysate
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab15503 被引用在 18 文献中.
- Astono IP et al. Objective quantification of nerves in immunohistochemistry specimens of thyroid cancer utilising deep learning. PLoS Comput Biol 18:e1009912 (2022). PubMed: 35226665
- Albayrak M et al. Prognostic value of cutaneous reinnervation with GAP-43 in oxaliplatin-induced neuropathy. J Neurol 269:4174-4184 (2022). PubMed: 35258850
- Semaniakou A et al. VIP reduction in the pancreas of F508del homozygous CF mice and early signs of Cystic Fibrosis Related Diabetes (CFRD). J Cyst Fibros 20:881-890 (2021). PubMed: 34034984
- Semaniakou A et al. Disrupted local innervation results in less VIP expression in CF mice tissues. J Cyst Fibros 20:154-164 (2021). PubMed: 32600901
- Ferdoushi A et al. Tumor innervation and clinical outcome in pancreatic cancer. Sci Rep 11:7390 (2021). PubMed: 33795769