AB109261

重组Anti-PGP9.5抗体[EPR4117]

Name: PGP9.5抗体[EPR4117] - Neuronal Marker (ab109261)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109261
Price: 3019 CNY
Availability: InStock
Rating: 3 (5 reviews)

Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker

RabMAb
Recombinant
KO Validated
了解详情

(5 Reviews)

(7 Publications)

Rabbit Recombinant Monoclonal PGP9.5 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

查看别名

Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, PGP9.5, UCHL1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : UCHL1 (KO) knockout HAP1 whole cell lysate (20 μg)
Lane 3 : SH-SY5Y whole cell lysate (20 μg)
Lane 4 : HEK293 whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (ab109261)

Predicted band size: 24 kDa

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AbReview27505****

Western blot - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

All lanes:

Western blot - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (ab109261) at 1/1000 dilution

Lane 1:

Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain) at 100 µg

Lane 2:

Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1) at 100 µg

Lane 3:

Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control) at 100 µg

Secondary

All lanes:

HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution

Predicted band size: 24 kDa

true

Exposure time: 2min

Image courtesy of Dr Simone Diestel by Abreview.

Unknown

Western blot - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

All lanes:

Western blot - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (ab109261) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Y79 lysate at 10 µg

Lane 3:

U-87 MG lysate at 10 µg

Lane 4:

SH SY5Y lysate at 10 µg

Predicted band size: 24 kDa

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OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] - Neuronal Marker (AB109261)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PGP9.5 antibody [EPR4117] - BSA and Azide free - Neuronal Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4117

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Perform antigen retrieval before commencing with IHC staining protocol", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Protein Gene Product 9.5 (PGP9.5) also known as ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is an enzyme of approximately 27 kDa mass. PGP9.5 is highly expressed in neurons and neuroendocrine cells and is involved in the processing of ubiquitinated proteins. Ubiquitination and deubiquitination are critical for maintaining cellular protein homeostasis. PGP9.5 is present in the central and peripheral nervous systems. Researchers often utilize PGP9.5 as a neuronal marker due to its strong expression in nerve tissues. Antibody clone 13C4 is commonly used in PGP9.5 immunohistochemistry to visualize this protein in histopathology sections.

Biological function summary

PGP9.5 plays a significant role in the ubiquitin-proteasome pathway where it regulates protein degradation. It functions by cleaving ubiquitin from ubiquitin-protein conjugates which can recycle ubiquitin for reuse impacting protein turnover. This process is important for removing misfolded or damaged proteins maintaining cellular integrity. PGP9.5 has not been documented as a part of any large multimeric complex; its action is rather individual yet vital in cellular processes.

Pathways

PGP9.5 is an important participant in the ubiquitin-dependent proteolysis pathway. This pathway controls the degradation of proteins that regulate cell cycle differentiation and neuromodulation. PGP9.5 works in tandem with other proteins such as the proteasome complex to remove proteins tagged for destruction. Another important pathway linked with PGP9.5 is the neuronal development pathway in which its activity supports neuron growth and repair.

Defective PGP9.5 function associates mainly with neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Mutations or dysregulation in PGP9.5 expression are implicated in the pathogenesis of these conditions affecting protein degradation and neuronal health. Other proteins like α-synuclein in Parkinson's disease interact with PGP9.5 pathways indicating their involvement in disease mechanisms. Researchers consider PGP9.5 a potential biomarker for neuronal pathology through methods like PGP9.5 IHC allowing for evaluation of nerve damage and neuroendocrine tumor diagnosis.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Deubiquitinase that plays a role in the regulation of several processes such as maintenance of synaptic function, cardiac function, inflammatory response or osteoclastogenesis (PubMed : 22212137, PubMed : 23359680). Abrogates the ubiquitination of multiple proteins including WWTR1/TAZ, EGFR, HIF1A and beta-site amyloid precursor protein cleaving enzyme 1/BACE1 (PubMed : 22212137, PubMed : 25615526). In addition, recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin to maintain a stable pool of monoubiquitin that is a key requirement for the ubiquitin-proteasome and the autophagy-lysosome pathways (PubMed : 12408865, PubMed : 8639624, PubMed : 9774100). Regulates amyloid precursor protein/APP processing by promoting BACE1 degradation resulting in decreased amyloid beta production (PubMed : 22212137). Plays a role in the immune response by regulating the ability of MHC I molecules to reach cross-presentation compartments competent for generating Ag-MHC I complexes (By similarity). Mediates the 'Lys-48'-linked deubiquitination of the transcriptional coactivator WWTR1/TAZ leading to its stabilization and inhibition of osteoclastogenesis (By similarity). Deubiquitinates and stabilizes epidermal growth factor receptor EGFR to prevent its degradation and to activate its downstream mediators (By similarity). Modulates oxidative activity in skeletal muscle by regulating key mitochondrial oxidative proteins (By similarity). Enhances the activity of hypoxia-inducible factor 1-alpha/HIF1A by abrogateing its VHL E3 ligase-mediated ubiquitination and consequently inhibiting its degradation (PubMed : 25615526).

See full target information UCHL1

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Science progress 108:368504251336287 PubMed40296549

2025

Suppression of SRC protein kinase activity alleviates the severity of aganglionosis by impairing CAV1/FLNA expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Xu,Yanqing Liu,Menglong Lan,Fei Liu,Huimin Xia,Jixiao Zeng

Biomedicines 10: PubMed36009539

2022

Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Yadav,Thamil Selvee Ramasamy,Sheng-Che Lin,Szu-Han Chen,Jean Lu,Ya-Hsin Liu,Fu-I Lu,Yuan-Yu Hsueh,Shau-Ping Lin,Chia-Ching Wu

Journal of neuroinflammation 18:238 PubMed34656124

2021

Sodium phenylbutyrate inhibits Schwann cell inflammation via HDAC and NFκB to promote axonal regeneration and remyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Anjali Yadav,Tzu-Chieh Huang,Szu-Han Chen,Thamil Selvee Ramasamy,Yuan-Yu Hsueh,Shau-Ping Lin,Fu-I Lu,Ya-Hsin Liu,Chia-Ching Wu

Experimental and therapeutic medicine 21:363 PubMed33732336

2021

Construction of differential expression plasmids of NGF to detect its influence on PC12 cell neuronal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Liu,Lili Zou,Peng Wang,Jingxuan Zhou,Chunling Yuan,Jun Wang

Journal of inflammation research 14:325-339 PubMed33584100

2021

Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Jiafei Cheng,Yiling Zhang,Jiande D Z Chen,Florin M Seralu

Journal of neuroinflammation 17:240 PubMed32799887

2020

Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Chieh Huang,Hua-Lin Wu,Szu-Han Chen,Yun-Ting Wang,Chia-Ching Wu

Global spine journal 3:201-18 PubMed24436871

2013

Notochordal cell-derived therapeutic strategies for discogenic back pain.

Applications

IHC

Species

Unspecified reactive species

D Purmessur,M C Cornejo,S K Cho,A C Hecht,J C Iatridis

View all publications

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重组Anti-PGP9.5抗体[EPR4117]