重组Anti-PGP9.5抗体[EPR4117] (ab109261)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-121981-anti-pgp95-antibody-epr4117-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4117] to PGP9.5
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PGP9.5抗体[EPR4117]
参阅全部 PGP9.5 一抗 -
描述
兔单克隆抗体[EPR4117] to PGP9.5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数（KD）
KD = 7.20 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR4117 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109261于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa).
|
| IHC-P | (3) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
靶标
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功能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
组织特异性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
疾病相关
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
序列相似性
Belongs to the peptidase C12 family. -
翻译后修饰
O-glycosylated. -
细胞定位
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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数据库链接
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q9R0P9 Mouse
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
see all -
别名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)
ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-279567-anti-pgp95-antibody-epr4117-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: UCHL1 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-121982-anti-pgp95-antibody-epr4117-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 lysate
Lane 3 : U-87 MG lysate
Lane 4 : SH SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa -
![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-121980-anti-pgp95-antibody-epr4117-western-blot.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)Image courtesy of Dr Simone Diestel by Abreview.All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 2 minutes
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![OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-121979-anti-pgp95-antibody-epr4117.jpg)
OI-RD Scanning - Anti-PGP9.5 antibody [EPR4117] (ab109261) -
![Anti-PGP9.5 antibody [EPR4117] (ab109261)](https://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-6-benefits-of-recombinant-antibodies.png)
Anti-PGP9.5 antibody [EPR4117] (ab109261)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab109261 被引用在 6 文献中.
- Yadav A et al. Autologous Platelet-Rich Growth Factor Reduces M1 Macrophages and Modulates Inflammatory Microenvironments to Promote Sciatic Nerve Regeneration. Biomedicines 10:N/A (2022). PubMed: 36009539
- Liu Y et al. Construction of differential expression plasmids of NGF to detect its influence on PC12 cell neuronal differentiation. Exp Ther Med 21:363 (2021). PubMed: 33732336
- Chen Y et al. Electroacupuncture at ST36 Relieves Visceral Hypersensitivity via the NGF/TrkA/TRPV1 Peripheral Afferent Pathway in a Rodent Model of Post-Inflammation Rectal Hypersensitivity. J Inflamm Res 14:325-339 (2021). PubMed: 33584100
- Yadav A et al. Sodium phenylbutyrate inhibits Schwann cell inflammation via HDAC and NF?B to promote axonal regeneration and remyelination. J Neuroinflammation 18:238 (2021). PubMed: 34656124
- Huang TC et al. Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching. J Neuroinflammation 17:240 (2020). PubMed: 32799887
- Purmessur D et al. Notochordal cell-derived therapeutic strategies for discogenic back pain. Global Spine J 3:201-18 (2013). IHC . PubMed: 24436871