AB188869

重组Anti-PGM1抗体[EPR15240]

Anti-PGM1 antibody [EPR15240]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal PGM1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

查看别名

Phosphoglucomutase-1, PGM 1, Glucose phosphomutase 1, PGM1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15240] (AB188869)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15240] (AB188869)

Immunofluorescent analysis of 293T cells (4% Paraformaldehyde-fixed) labeling PGM1 with ab188869 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] (AB188869)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PGM1 antibody [EPR15240] (AB188869)

Intracellular flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-PGM1 antibody [EPR15240] (AB188869)

Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-PGM1 antibody [EPR15240] (ab188869)

Predicted band size: 61 kDa

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Lab

Western blot - Anti-PGM1 antibody [EPR15240] (AB188869)

Western blot : Anti-PGM1 antibody [EPR15240] ab188869 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 68 kDa in Wild-type A549 cell lysates with no signal observed at this size in PGM1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

PGM1 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

K562 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 61 kDa

Observed band size: 68 kDa,37 kDa

false

Supplier Data

Western blot - Anti-PGM1 antibody [EPR15240] (AB188869)

All lanes:

Western blot - Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal liver lysate at 10 µg

Lane 3:

293T lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution

Predicted band size: 61 kDa

false

Supplier Data

Western blot - Anti-PGM1 antibody [EPR15240] (AB188869)

All lanes:

Western blot - Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 61 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-PGM1 antibody [EPR15240] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15240

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, ICC/IF, Flow Cyt (Intra), IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/360", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Protein PGM1 also known as phosphoglucomutase-1 plays a significant mechanical role in cellular glucose metabolism. It catalyzes the interconversion of glucose-1-phosphate and glucose-6-phosphate a critical step in the glycolysis and gluconeogenesis pathways. The PGM1 protein has a molecular mass of approximately 61 kDa. Its expression occurs in various tissues with high levels in muscle and liver tissues which are key sites for energy storage and utilization.

Biological function summary

PGM1 contributes significantly to both energy homeostasis and carbohydrate metabolism. It functions as part of a dynamic complex involving other enzymes that manage intracellular energy balance. PGM1 supports glycogen synthesis and breakdown by regulating the availability of glucose-1-phosphate. This dual role makes PGM1 an important protein for maintaining normal metabolic function. Its activity impacts cellular energy levels which is vital for muscle contraction and overall energy distribution in tissues.

Pathways

PGM1 is critically involved in carbohydrate metabolic pathways specifically glycolysis and gluconeogenesis. In glycolysis it participates in converting glucose for energy production. In gluconeogenesis it assists in the generation of glucose from non-carbohydrate substrates. PGM1 interacts closely with other enzymes in these pathways such as glucose-6-phosphate dehydrogenase ensuring efficient energy regulation and metabolic flow within the cell.

PGM1 is associated with glycogen storage disease type XIV which is a condition causing improper glycogen metabolism. Mutations in the PGM1 gene disrupt its enzymatic function leading to symptoms like muscle pain and weakness. Additionally PGM1 defects can impact another protein phosphoglucomutase-3 (PGM3) which shares pathway similarities and potentially affects the synthesis of other phosphorylated sugars related to immune function. These connections highlight the importance of functional PGM1 in maintaining normal physiological and disease-free states.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the reversible isomerization of alpha-D-glucose 1-phosphate to alpha-D-glucose 6-phosphate (PubMed : 15378030, PubMed : 25288802). The mechanism proceeds via the intermediate compound alpha-D-glucose 1,6-bisphosphate (Probable) (PubMed : 25288802). This enzyme participates in both the breakdown and synthesis of glucose (PubMed : 17924679, PubMed : 25288802).

See full target information PGM1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Bone research 6:32 PubMed30455992

2018

NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Ye,Feng Lou,Fanyuan Yu,Demao Zhang,Chenglin Wang,Fanzi Wu,Xin Li,Yilin Ping,Xiao Yang,Jing Yang,Dian Chen,Bo Gao,Dingming Huang,Peng Liu

View all publications

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重组Anti-PGM1抗体[EPR15240]