AB113687

Anti-PGK1 抗体 [22C5D8]

Name: PGK1抗体[22C5D8] (ab113687)| Abcam中文官网
Brand: Abcam Limited
SKU: AB113687
Price: 4116 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-PGK1 antibody [22C5D8]

(1 Review)

(188 Publications)

Anti-PGK1 antibody [22C5D8] (ab113687) is a mouse monoclonal antibody detecting PGK1 in Western Blot. Suitable for Saccharomyces cerevisiae.

- Over 140 publications

查看别名

YCR012W, YCR12W, PGK1, Phosphoglycerate kinase

2 Images

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Western blot - Anti-PGK1 antibody [22C5D8] (AB113687)

All lanes:

Western blot - Anti-PGK1 antibody [22C5D8] (ab113687)

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Yeast whole cell lysate at 15 µg

Predicted band size: 45 kDa

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AbReview60407****

Western blot - Anti-PGK1 antibody [22C5D8] (AB113687)

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration : 5% · Temperature : 25°C.

Gel running condition : Reduced Denaturing (4-12% Bis-Tris).

All lanes:

Western blot - Anti-PGK1 antibody [22C5D8] (ab113687) at 1/1000 dilution

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Saccharomyces cerevisiae Cell lysate - nuclear at 30 µg

Secondary

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Sheep anti-Mouse IgG, HRP-linked whole Ab ECL at 1/5000 dilution

Predicted band size: 45 kDa

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Exposure time: 1min

This image is courtesy of an anonymous abreview.

不同偶联物与剂型 (1)

HRP

HRP Anti-PGK1 antibody [22C5D8]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

22C5D8

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Saccharomyces cerevisiae

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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产品详情

What is this antibody validated in?
Anti-PGK1 antibody [22C5D8] (ab113687) is a mouse monoclonal antibody and is validated for use in Western Blot (WB) in Saccharomyces cerevisiae samples.

What is the molecular weight of PGK1?
Anti-PGK1 [22C5D8] (ab113687) specifically detects a band for PGK1 (UniProt: P00558) at a molecular weight of 45kDa.

Trusted by the scientific community
Anti-PGK1 [22C5D8] (ab113687) was first used in a scientific publication in 2011 and has been cited over 140 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [22C5D8] also available for your convenience: ab113687, HRP - ab197960

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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性能和储存信息

形式

Liquid

纯化工艺

Proprietary technique

纯化说明

Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.

存储溶液

pH: 7.5 Preservative: 0.02% Sodium azide Constituents: HEPES buffered saline

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PGK1 also known as phosphoglycerate kinase-1 is an enzyme involved in glycolysis with a mass of approximately 45 kDa. PGK1 catalyzes the reversible transfer of a phosphate group from 13-bisphosphoglycerate to ADP forming 3-phosphoglycerate and ATP. This enzyme is expressed ubiquitously across various tissues with significant roles in energy metabolism. In yeast it is referred to simply as phosphoglycerate kinase where its function in cellular respiration is widely studied.

Biological function summary

The PGK1 protein plays an essential role in energy production by participating in the glycolytic pathway. This enzyme functions independently and is not part of any complex. By converting 13-bisphosphoglycerate to 3-phosphoglycerate it supports cellular processes that require ATP. Such activity facilitates the high energy needs of cells ensuring proper cellular function and homeostasis.

Pathways

The enzyme PGK1 operates predominantly within the glycolytic and gluconeogenesis pathways. Throughout glycolysis it partners with enzymes like phosphofructokinase and pyruvate kinase facilitating the breakdown of glucose into pyruvate while generating ATP. In gluconeogenesis PGK1 aids in glucose formation which helps balance energy dynamics particularly in tissues like the liver and kidney.

PGK1 has associations with tumor growth and proliferation in various cancers. Its elevated expression is often found in tumors linking it to oncogenesis due to the increased demand for glycolytic activity. Additionally mutations in PGK1 can result in phosphoglycerate kinase deficiency a disorder affecting red blood cell metabolism. This links it to related proteins involved in managing metabolic pathways of energy production in affected tissues.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate (PubMed : 6254992). Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines (By similarity). Negatively regulates the biosynthesis of acetyl-CoA from pyruvate in the mitochondrion (By similarity).

See full target information PGK1

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文献 (188)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7212 PubMed40764615

2025

Arp2/3 and type-I myosins control chromosome mobility and end-resection at double-strand breaks in S. cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Felix Y Zhou,Marissa Ashton,Yiyang Jiang,Neha Arora,Kevin Clark,Kate B Fitzpatrick,James E Haber

Nature communications 16:5314 PubMed40595511

2025

A constricted mitochondrial morphology formed during respiration.

Applications

Unspecified application

Species

Unspecified reactive species

Manish K Singh,Laetitia Cavellini,Maria Angeles Morcillo-Parra,Christina Kunz,Mickaël Lelek,Perrine Bomme,Aurélia Barascu,Cynthia Alsayyah,Maria Teresa Teixeira,Naïma Belgareh-Touzé,Adeline Mallet,Lea Dietrich,Christophe Zimmer,Mickael M Cohen

Protein science : a publication of the Protein Society 34:e70207 PubMed40545685

2025

Ancestral sequence reconstruction of the Mic60 Mitofilin domain reveals residues supporting respiration in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Friederike M C Benning,Tristan A Bell,Tran H Nguyen,Della Syau,Louise B Connell,Yi-Ting Liao,Matthew P Keating,Margaret Coughlin,Anja E H Nordstrom,Maria Ericsson,Corrie J B daCosta,Luke H Chao

Genetics 231: PubMed40546018

2025

Revealing function-altering MECP2 mutations in individuals with autism spectrum disorder using yeast and Drosophila.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Chen,Jessica Schmitt,Graeme McIntosh,Barry P Young,Tianshun Lian,Jie Liu,Kexin K Chen,J Beatrice Liston,Lily MacDonald,Bill Wang,Sonia Medina Giro,Benjamin Boehme,Mriga Das,Seevasant Indran,Jesse T Chao,Sanja Rogic,Paul Pavlidis,Douglas W Allan,Christopher J R Loewen

Life science alliance 8: PubMed40541417

2025

Hydroxyurea modulates thiol-disulfide homeostasis in the yeast endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Takano,Yuki Ishiwata-Kimata,Ryo Ushioda,Yukio Kimata,Kunio Nakatsukasa

microPublication biology 2025: PubMed40406580

2025

Pep12 is important for proteasome microautophagy under low glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Kyle VanderVen,Conner Butcher,Remi' Fokine,Jianhui Li

Frontiers in cell and developmental biology 13:1554958 PubMed40365021

2025

Omic AI reveals new autophagy regulators from the Atg1 interactome in .

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Han,Shanshan Fu,Dachao Tang,Yuting Chen,Dan Liu,Zihao Feng,Yujie Gou,Chi Zhang,Weizhi Zhang,Leming Xiao,Jiayi Zhang,Cong Yi,Yu Xue,Di Peng

PLoS biology 23:e3003111 PubMed40208903

2025

Phase separation of the PRPP amidotransferase into dynamic condensates promotes de novo purine synthesis in yeast.

Applications

Unspecified application

Species

Unspecified reactive species

Masak Takaine,Rikuri Morita,Yuto Yoshinari,Takashi Nishimura

Genetics 229: PubMed40178993

2025

A new set of mutations in the second transmembrane helix of the Cox2p-W56R substantially improves its allotopic expression in Saccharomyces cerevisiae.

Applications

Unspecified application

Species

Unspecified reactive species

Kewin Gombeau,Stefan A Hoffmann,Yizhi Cai

Nature structural & molecular biology 32:1006-1018 PubMed39930008

2025

Initiation of ERAD by the bifunctional complex of Mnl1/Htm1 mannosidase and protein disulfide isomerase.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Zhao,Xudong Wu,Tom A Rapoport

View all publications

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Anti-PGK1 抗体 [22C5D8]