AB129199

重组Anti-PGD抗体[EPR6565]

Anti-PGD antibody [EPR6565]

RabMAb
Recombinant
了解详情

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(15 Publications)

Rabbit Recombinant Monoclonal PGD antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 15 publications.

查看别名

PGDH, PGD

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] (AB129199)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PGD antibody [EPR6565] (AB129199)

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PGD with ab129199 at 1/500. Cells were fixed with 100% methanol. ab150077 an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control : PBS only.
Nuclear counter stain : DAPI.

Lab

Western blot - Anti-PGD antibody [EPR6565] (AB129199)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PGD antibody [EPR6565] (ab129199) at 1/5000 dilution

Lane 1:

Mouse brain tissue lysates at 20 µg

Lane 2:

Mouse heart tissue lysates at 20 µg

Lane 3:

Mouse kidney tissue lysates at 20 µg

Lane 4:

Mouse spleen tissue lysates at 20 µg

Lane 5:

Rat brain tissue lysates at 20 µg

Lane 6:

Rat heart tissue lysates at 20 µg

Lane 7:

Rat kidney tissue lysates at 20 µg

Lane 8:

Rat spleen tissue lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 45 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-PGD antibody [EPR6565] (AB129199)

All lanes:

Western blot - Anti-PGD antibody [EPR6565] (ab129199) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Molt-4 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 53 kDa

Observed band size: 45 kDa

false

OI-RD Scanning - Anti-PGD antibody [EPR6565] (AB129199)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PGD antibody [EPR6565] (AB129199)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PGD antibody [EPR6565] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6565

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PGD also known as 6-phosphogluconate dehydrogenase is an enzyme with a mass of approximately 53 kDa. It plays an important role in catalyzing the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate producing NADPH in the process. PGD expression occurs in various tissues with notably high levels in liver and red blood cells (RBCs). Alternative names include 6PGD and phosphogluconate dehydrogenase with its corresponding gene expressed across eukaryotic organisms.

Biological function summary

The enzyme 6-phosphogluconate dehydrogenase is essential in cellular metabolism and redox homeostasis. It functions as part of the pentose phosphate pathway a metabolic route critical for the generation of NADPH and ribose 5-phosphate. NADPH is necessary for biosynthetic reactions and in protecting cells against oxidative stress. 6PGD operates as a homodimer and is not part of a larger protein complex enabling it to perform its functions independently within the cell.

Pathways

PGD stands as a major component of the pentose phosphate pathway alongside glucose-6-phosphate dehydrogenase (G6PD). This pathway is significant for providing reducing power and pentose phosphates for anabolic reactions. Both 6-phosphogluconate and G6PD assist in the proper functioning of this pathway facilitating the balance of NADP+/NADPH. The pathway intricately connects to glycolysis enabling the cells to adapt to fluctuations in metabolic demands through intermediates that interconvert between these pathways.

Altered activity of 6-phosphogluconate dehydrogenase links to genetic disorders like hemolytic anemia. The pentose phosphate pathway's impairment particularly due to deficiency in PGD or its associated protein G6PD exacerbates anemia under oxidative stress. Additionally PGD overexpression has associations with many cancers as elevated NADPH production supports tumor survival and resistance. Understanding the role of PGD in these conditions helps in recognizing the importance of this enzyme in maintaining cellular health and adapting to disease challenges.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO(2), with concomitant reduction of NADP to NADPH.

See full target information PGD

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文献 (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1001 PubMed40993625

2025

Triclabendazole inhibits PKM2 nuclear localization and glycolysis by enhancing HDAC6-mediated deacetylation in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Yan,Yong Sun,Shan-Shan Shi,Yuan Li,Yi-Fan Zhang,Liang-Zhuo Qu,Jing Liu,Yong Dai,Qing-Bing Zha,Jun Fan

EMBO reports 26:3384-3410 PubMed40437287

2025

KEAP1 retention in phase-separated p62 bodies drives liver damage under autophagy-deficient conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhei Takada,Nozomi Shinomiya,Gaoxin Mao,Hikaru Tsuchiya,Tomoaki Koga,Satoko Komatsu-Hirota,Yu-Shin Sou,Manabu Abe,Elena Ryzhii,Michitaka Suzuki,Mitsuyoshi Nakao,Satoshi Waguri,Hideaki Morishita,Masaaki Komatsu

Metabolites 14: PubMed39195531

2024

Metabolic and Oxidative Stress Management Heterogeneity in a Panel of Breast Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Maycotte,Fabiola Lilí Sarmiento-Salinas,Alin García-Miranda,Cesar Ivan Ovando-Ovando,Diana Xochiquetzal Robledo-Cadena,Luz Hernández-Esquivel,Ricardo Jasso-Chávez,Alvaro Marín-Hernández

JCI insight 9: PubMed38452174

2024

Differentially disrupted spinal cord and muscle energy metabolism in spinal and bulbar muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle DeBartolo,Frederick J Arnold,Yuhong Liu,Elana Molotsky,Hsin-Yao Tang,Diane E Merry

Nature cell biology 24:148-154 PubMed35165416

2022

Metabolic determination of cell fate through selective inheritance of mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Döhla,Emilia Kuuluvainen,Nadja Gebert,Ana Amaral,Johanna I Englund,Swetha Gopalakrishnan,Svetlana Konovalova,Anni I Nieminen,Ella S Salminen,Rubén Torregrosa Muñumer,Kati Ahlqvist,Yang Yang,Hien Bui,Timo Otonkoski,Reijo Käkelä,Ville Hietakangas,Henna Tyynismaa,Alessandro Ori,Pekka Katajisto

Nature metabolism 4:123-140 PubMed35102339

2022

Oxidative pentose phosphate pathway controls vascular mural cell coverage by regulating extracellular matrix composition.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola Facchinello,Matteo Astone,Matteo Audano,Roxana E Oberkersch,Marianna Spizzotin,Enrica Calura,Madalena Marques,Mihaela Crisan,Nico Mitro,Massimo M Santoro

Journal of molecular and cellular cardiology 164:136-147 PubMed34923199

2021

A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Giulia Emanuelli,Anna Zoccarato,Christina M Reumiller,Angelos Papadopoulos,Mei Chong,Sabine Rebs,Kai Betteridge,Matteo Beretta,Katrin Streckfuss-Bömeke,Ajay M Shah

JCI insight : PubMed33497360

2021

The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonates.

Applications

Unspecified application

Species

Unspecified reactive species

Jiannan Gong,Zihang Feng,Abigail L Peterson,Jennifer F Carr,Xuexin Lu,Haifeng Zhao,Xiangming Ji,You-Yang Zhao,Monique E De Paepe,Phyllis A Dennery,Hongwei Yao

Cell death & disease 11:586 PubMed32719331

2020

HIF-1α regulates cellular metabolism, and Imatinib resistance by targeting phosphogluconate dehydrogenase in gastrointestinal stromal tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Kangjing Xu,Zhongyuan He,Ming Chen,Nuofan Wang,Diancai Zhang,Li Yang,Zekuan Xu,Hao Xu

Cell biology international 44:137-144 PubMed31393075

2019

Up-regulation of the pentose phosphate pathway and HIF-1α expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiro Tokuda,Byron Baron,Chiemi Yamashiro,Yasuhiro Kuramitsu,Takao Kitagawa,Masaaki Kobayashi,Koh-Hei Sonoda,Kazuhiro Kimura

View all publications

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重组Anti-PGD抗体[EPR6565]