重组Anti-PGC1 beta抗体[EPR12370] (ab176328)
![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.cn/ps/products/176/ab176328/Images/ab176328-257234-anti-pgc1-beta-antibody-epr12370-western-blot.jpg "Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12370] to PGC1 beta
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PGC1 beta抗体[EPR12370]
参阅全部 PGC1 beta 一抗 -
描述
兔单克隆抗体[EPR12370] to PGC1 beta -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt,ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: HeLa, MCF-7 and SH-SY5Y cell lysates and human fetal brain, human fetal heart, rat spleen and mouse spleen tissue lysates. IP: HeLa whole cell lysate (ab150035).
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常规说明
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12370 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176328于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 113 kDa.
|
| IP |
1/10 - 1/100.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 113 kDa. |
|
IP
1/10 - 1/100. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
靶标
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功能
Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. -
组织特异性
Ubiquitous with higher expression in heart, brain and skeletal muscle. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
结构域
Contains 2 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motif, which are usually required for the association with nuclear receptors. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 291567 Rat
- Omim: 608886 Human
- SwissProt: Q86YN6 Human
- SwissProt: Q8VHJ7 Mouse
- SwissProt: Q811R2 Rat
- Unigene: 483816 Human
see all -
别名
- PERC antibody
- peroxisome proliferative activated receptor, gamma, coactivator 1, antibody
- peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
see all
图片
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Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : SH-SY5Y whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.cn/ps/products/176/ab176328/Images/ab176328-257235-anti-pgc1-beta-antibody-epr12370-western-blot.jpg)
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/10000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 113 kDa
Observed band size: 113 kDa
Blocking and dilution buffer: 5% NFDM/TBST. -
![Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.cn/ps/products/176/ab176328/Images/ab176328-257199-anti-pgc1-beta-antibody-epr12370-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)ab176328 (purified) at 1/20 immunoprecipitating PGC1 beta in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab176328 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab176328 in HeLa whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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![Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.cn/ps/products/176/ab176328/Images/ab176328-204140-anti-pgc1-beta-antibody-epr12370-western-blot.jpg)
Western blot - Anti-PGC1 beta antibody [EPR12370] (ab176328)All lanes : Anti-PGC1 beta antibody [EPR12370] (ab176328) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Human fetal heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa -
![Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.cn/ps/products/176/ab176328/Images/ab176328-204143-anti-pgc1-beta-antibody-epr12370-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PGC1 beta antibody [EPR12370] (ab176328)Western blot analysis on immunoprecipitation pellet from HeLa cell lysate labeling PGC1 beta using unpurified ab176328 at a dilution of 1/10.
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![Anti-PGC1 beta antibody [EPR12370] (ab176328)](https://www.abcam.cn/ps/products/176/ab176328/Images/ab176328-8-benefits-of-recombinant-antibodies.png)
Anti-PGC1 beta antibody [EPR12370] (ab176328)
数据表及文件
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SDS download
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Datasheet download
文献 (48)
ab176328 被引用在 48 文献中.
- Yin X et al. Deficiency of lipopolysaccharide binding protein facilitates adipose browning, glucose uptake and oxygen consumption in mouse embryonic fibroblasts via activating PI3K/Akt/mTOR pathway and inhibiting autophagy. Cell Cycle 22:967-985 (2023). PubMed: 36710409
- Zhen C et al. Ganoderma lucidum polysaccharides attenuates pressure-overload-induced pathological cardiac hypertrophy. Front Pharmacol 14:1127123 (2023). PubMed: 37033616
- Paladines N et al. Metabolic reprogramming through mitochondrial biogenesis drives adenosine anti-inflammatory effects: new mechanism controlling gingival fibroblast hyper-inflammatory state. Front Immunol 14:1148216 (2023). PubMed: 37350964
- Deng F et al. Dulaglutide Protects Mice against Diabetic Sarcopenia-Mediated Muscle Injury by Inhibiting Inflammation and Regulating the Differentiation of Myoblasts. Int J Endocrinol 2023:9926462 (2023). PubMed: 37584041
- Arisumi S et al. Metallothionein 3 promotes osteoclast differentiation and survival by regulating the intracellular Zn2+ concentration and NRF2 pathway. Cell Death Discov 9:436 (2023). PubMed: 38040717