AB72230

Anti-PGC1 alpha + beta抗体

Name: PGC1 alpha + beta抗体 (ab72230)| Abcam中文官网
Brand: Abcam Limited
SKU: AB72230
Price: 4047 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-PGC1 alpha + beta antibody

(2 Reviews)

(52 Publications)

Rabbit Polyclonal PGC1 alpha antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide - Human, Mouse, Rat, Human samples. Cited in 52 publications.

查看别名

peroxisome proliferator-activated receptor gamma coactivator 1 beta, Peroxisome proliferative activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, peroxisome proliferator-activated receptor gamma, coactivator 1 beta, Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT, Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext, LEM6, PERC, PGC1, PGC1A, PGC1v, PGC 1v, PPARGC1, PPARGC1A, Ppargc1b, PGC 1 alpha, PGC-1(beta), PGC-1-alpha, PGC-1-beta, PGC1(alpha), PRGC1_HUMAN, L PGC 1alpha, PRGC2_HUMAN, PGC 1 (alpha), PPARGC 1 alpha, PPARGC-1-alpha, PPARGC-1-beta, PPAR gamma coactivator 1, Ligand effect modulator 6, PPAR gamma coactivator-1beta, PPAR gamma coactivator 1 alpha, PPAR-gamma coactivator 1-alpha, PPAR-gamma coactivator 1-beta, PPAR gamma coactivator variant form, PGC-1-related estrogen receptor alpha coactivator, PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6, Peroxisome proliferative activated receptor gamma coactivator 1, Peroxisome proliferative activated receptor, gamma, coactivator 1, Peroxisome proliferator activated receptor gamma coactivator 1 alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-beta

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling PGC1 alpha+beta with ab72230 at a 1/100 dilution showing nuclear staining. Permeabilisation using 0.1% tritonX-100.

Secondary ab : Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/1000 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab72230 at 1/100 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. -ve control 2 is ab7291 at 1/1000 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

Unknown

Western blot - Anti-PGC1 alpha + beta antibody (AB72230)

Western Blot showing equal blocking with the PGC1 alpha and PGC1 beta peptides.

All lanes:

Western blot - Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/mL

Lane 1:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 2:

Colon (Mouse) Tissue Lysate at 10 µg

Lane 3:

Thymus (Rat) Tissue Lysate at 10 µg

Lane 4:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg with Human PGC1 alpha peptide (<a href='/products/unavailable/human-pgc1-alpha-peptide-ab73600'>ab73600</a>)

Lane 5:

Colon (Mouse) Tissue Lysate at 10 µg with Human PGC1 alpha peptide (<a href='/products/unavailable/human-pgc1-alpha-peptide-ab73600'>ab73600</a>)

Lane 6:

Thymus (Rat) Tissue Lysate at 10 µg with Human PGC1 alpha peptide (<a href='/products/unavailable/human-pgc1-alpha-peptide-ab73600'>ab73600</a>)

Lane 7:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg with PGC1 beta peptide

Lane 8:

Colon (Mouse) Tissue Lysate at 10 µg with PGC1 beta peptide

Lane 9:

Thymus (Rat) Tissue Lysate at 10 µg with PGC1 beta peptide

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 91 kDa

Observed band size: 110 kDa,45 kDa,73 kDa,90 kDa

false

Exposure time: 8min

Project6386****

Western blot - Anti-PGC1 alpha + beta antibody (AB72230)

We have occasionally observed bands in the region of 90 kDa in batches of this antibody, which corresponds to the molecular weight based on primary sequence.

All lanes:

Western blot - Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/mL

Lane 1:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 2:

Human skeletal muscle tissue lysate - total protein (<a href='/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 3:

Ovary (Rat) Tissue Lysate at 10 µg

Lane 4:

Colon (Mouse) Tissue Lysate at 10 µg

Lane 5:

Skeletal Muscle (Mouse) Tissue Lysate at 10 µg

Lane 6:

Thymus (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 91 kDa

Observed band size: 110 kDa,35 kDa,45 kDa,55 kDa

true

Exposure time: 2min

ELISA - Anti-PGC1 alpha + beta antibody (AB72230)

ELISA

Lab

ELISA - Anti-PGC1 alpha + beta antibody (AB72230)

ab72230 was tested using an Indirect ELISA approach. The wells were coated with peptide (1μg/ml at 100μl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025μg/ml (100μl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1 : 20,000 dilution for 1hr at room temperature.

ab72230 gave a positive result in ELISA against PGC1 alpha peptide and PGC1 beta peptide. This indicates that it recognises both forms.

Orange line : ELISA against immunising peptide ab73600
Yellow line : ELISA against ab88232

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, ELISA, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen design for ab72230 is shown to cross-react with both PGC1 alpha (ID: 124782 and Swissprot Q9UBK2) and PGC1 beta (ID: 240776 and SwissProt Q86YN6). From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab188102.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Chicken": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "0.25-1000 ng/mL", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

PGC1 alpha has a predicted MW of 92 kDa and PGC1 beta has a predicted MW of 113 kDa.

This product may be covered by one or more of the following U.S. Patents.

6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PGC1 alpha and beta also known as Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha (PGC-1α) and 1-beta (PGC-1β) are transcriptional coactivators with molecular weights of approximately 91 kDa and 102 kDa respectively. These proteins are important in regulating energy metabolism and are highly expressed in tissues with high energy demand such as skeletal muscle heart and brown adipose tissue. The proteins interact with nuclear hormone receptors and various transcription factors to control the expression of genes involved in energy homeostasis.

Biological function summary

PGC1 alpha and beta activate and coordinate the expression of genes involved in mitochondrial biogenesis and oxidative phosphorylation. They do not form a complex themselves but work with other transcriptional regulators to enhance gene expression. These proteins influence the metabolic processes of cells regulating the conversion of energy substrates into usable cellular energy. They are critical in adapting to different physiological conditions like exercise and fasting by modulating the capacity for energy production.

Pathways

PGC1 alpha and beta integrate signals from various pathways like the PPAR and AMPK pathways which are significant for energy balance and metabolism. PGC1 alpha particularly interacts with proteins such as NRF1 and NRF2 to promote mitochondrial function. Through these pathways PGC1 coactivators help regulate metabolism by altering the transcriptional activity of genes involved in energy expenditure and metabolic adaptation.

PGC1 alpha and beta have significant implications in conditions such as obesity and diabetes. Dysregulation of these proteins affects the balance of energy metabolism leading to metabolic disorders. PGC1 alpha in particular interacts with proteins like SIRT1 which influence insulin sensitivity and metabolic rate thereby playing a role in the development and progression of these diseases. The modulation of PGC1 activity has potential therapeutic implications for managing metabolic disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional coactivator for steroid receptors and nuclear receptors (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism (PubMed : 10713165, PubMed : 20005308, PubMed : 21376232). Acts as a key regulator of gluconeogenesis : stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program (PubMed : 16753578, PubMed : 23142079). Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner (PubMed : 23836911). Also involved in the integration of the circadian rhythms and energy metabolism (By similarity). Required for oscillatory expression of clock genes, such as BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).

See full target information PPARGC1A

其他靶点

PPARGC1B

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文献 (52)

Recent publications for all applications. Explore the full list and refine your search

Life metabolism 4:loaf015 PubMed40585527

2025

Baf60c in skeletal muscle regulates adipose tissue thermogenesis via Musclin-mediated endocrine signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Han,Lu Jin,Wei Peng,Xue Lv,Ziyin Zhang,Tongyu Liu,Lin Mi,Yue Gao,Jun-Fen Fu,Zhuo-Xian Meng

Animals : an open access journal from MDPI 15: PubMed39943061

2025

Diosmetin Delays In Vitro Aging of Porcine Oocytes by Improving Mitochondrial Function and Reducing Oxidative Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Jun Ren,Xiu-Wen Yuan,Yu-Hao Zhang,Zi-Long Meng,Xing-Wei Liang,Nam-Hyung Kim,Yong-Nan Xu,Ying-Hua Li

Veterinary sciences 11: PubMed39728965

2024

Effects of Caffeic Acid Phenethyl Ester on Embryonic Development Through Regulation of Mitochondria and Endoplasmic Reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Chu-Man Huang,Hui-Mei Huang,Ying-Hua Li,Xing-Wei Liang,Nam-Hyung Kim,Yong-Nan Xu

Autism research : official journal of the International Society for Autism Research 18:933-953 PubMed39611328

2024

Analysis of mitochondrial DNA replisome in autism spectrum disorder: Exploring the role of replisome genes.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Rojas,Carlos Carrasco-Gallardo,Lidia Tenorio,Margrethe A Olesen,Victor Tapia,Manuel Carrasco,Patricio Araos,Rodrigo A Quintanilla,Lina M Ruiz

Biomolecules 14: PubMed39595642

2024

Methyl Paraben Affects Porcine Oocyte Maturation Through Mitochondrial Dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Huimei Huang,Chuman Huang,Yinghua Li,Xingwei Liang,Namhyung Kim,Yongnan Xu

Drug design, development and therapy 18:699-717 PubMed38465266

2024

Integrated Network Pharmacology and in vivo Experimental Validation Approach to Explore the Potential Antioxidant Effects of Annao Pingchong Decoction in Intracerebral Hemorrhage Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xuqing Zhou,Xu Wang,Jiaqi Li,Mengxue Zhang,Yi Yang,Shihui Lei,Ying He,Hua Yang,Desheng Zhou,Chun Guo

Cell chemical biology 30:1585-1600.e6 PubMed37890479

2023

Blocking AMPKαS496 phosphorylation improves mitochondrial dynamics and hyperglycemia in aging and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Alexia Pearah,Balamurugan Ramatchandirin,Ting Liu,Risa M Wolf,Arisa Ikeda,Sally Radovick,Hiromi Sesaki,Fredric E Wondisford,Brian O'Rourke,Ling He

Science advances 9:eadi2767 PubMed37624892

2023

ANT-dependent MPTP underlies necrotic myofiber death in muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Bround,Julian R Havens,Allen J York,Michelle A Sargent,Jason Karch,Jeffery D Molkentin

American journal of physiology. Regulatory, integrative and comparative physiology 325:R490-R503 PubMed37545421

2023

Clock genes regulate skeletal muscle energy metabolism through NAMPT/NAD/SIRT1 following heavy-load exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xia,Binyu Yao,Zeting Fu,Lunyu Li,Songlin Jin,Bo Qu,Ying Huang,Haili Ding

Nature communications 14:4257 PubMed37468484

2023

The muscle-enriched myokine Musclin impairs beige fat thermogenesis and systemic energy homeostasis via Tfr1/PKA signaling in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Jin,Shuang Han,Xue Lv,Xiaofei Li,Ziyin Zhang,Henry Kuang,Zhimin Chen,Cheng-An Lv,Wei Peng,Zhuoying Yang,Miqi Yang,Lin Mi,Tongyu Liu,Shengshan Ma,Xinyuan Qiu,Qintao Wang,Xiaowen Pan,Pengfei Shan,Yu Feng,Jin Li,Fudi Wang,Liwei Xie,Xuyun Zhao,Jun-Fen Fu,Jiandie D Lin,Zhuo-Xian Meng

View all publications

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Abcam Product Promise

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For licensing inquiries, please contact partnerships@abcam.com

Anti-PGC1 alpha + beta抗体

Anti-PGC1 alpha + beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-PGC1 alpha + beta antibody (AB72230)

Western blot - Anti-PGC1 alpha + beta antibody (AB72230)

Western blot - Anti-PGC1 alpha + beta antibody (AB72230)

ELISA - Anti-PGC1 alpha + beta antibody (AB72230)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性