Anti-PGC1 alpha抗体[4A8] (ab77210)
![Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210)](https://www.abcam.cn/ps/products/77/ab77210/Images/PGC1-alpha-Primary-antibodies-ab77210-1.jpg "Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210)")
Key features and details
- Mouse monoclonal [4A8] to PGC1 alpha
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
概述
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产品名称
Anti-PGC1 alpha抗体[4A8]
参阅全部 PGC1 alpha 一抗 -
描述
小鼠单克隆抗体[4A8] to PGC1 alpha -
宿主
Mouse -
特异性
The immunogen sequence for this antibody is 55% homologous with PGC1 beta (Uniprot: Q86YN6). -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human PGC1 alpha aa 689-799.
Database link: Q9UBK2 -
阳性对照
- PGC1 alpha transfected U2OS cell lysate
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常规说明
This product was changed from ascites to tissue culture supernatant on 12/3/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Constituents: PBS, 8% Sodium chloride, 0.6% Dibasic monohydrogen sodium phosphate, 0.2% Monobasic dihydrogen potassium phosphate, 0.2% Potassium chloride -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
4A8 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Nucleotide metabolism
- Molecular processes
- Mitochondrial transcription
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77210于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). |
|
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. -
组织特异性
Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain. -
翻译后修饰
Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body. - Information by UniProt
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数据库链接
- Entrez Gene: 10891 Human
- Omim: 604517 Human
- SwissProt: Q9UBK2 Human
- Unigene: 527078 Human
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别名
- L PGC 1alpha antibody
- LEM6 antibody
- Ligand effect modulator 6 antibody
see all
图片
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Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210)Anti-PGC1 alpha antibody [4A8] (ab77210) at 1 µg/ml + Recombinant fragment of human PGC1 alpha at 0.1 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?Western blot against tagged recombinant protein immunogen using ab77210 antibody at 1/500 dilution. Predicted band size of immunogen is 92kDa.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-PGC1 alpha antibody [4A8] (ab77210)](https://www.abcam.cn/ps/products/77/ab77210/Images/PGC1-alpha-Primary-antibodies-ab77210-3.jpg)
Flow Cytometry - Anti-PGC1 alpha antibody [4A8] (ab77210)Overlay histogram showing HEK293 cells stained with ab77210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77210, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
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![Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210)](https://www.abcam.cn/ps/products/77/ab77210/Images/PGC1-alpha-Primary-antibodies-ab77210-2.jpg)
Western blot - Anti-PGC1 alpha antibody [4A8] (ab77210)Anti-PGC1 alpha antibody [4A8] (ab77210) at 1 µg/ml + PGC1 alpha transfected U2OS cell lysate at 25 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
数据表及文件
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Datasheet download
文献 (17)
ab77210 被引用在 17 文献中.
- Longhitano L et al. (+)-Lipoic Acid Reduces Lipotoxicity and Regulates Mitochondrial Homeostasis and Energy Balance in an In Vitro Model of Liver Steatosis. Int J Mol Sci 24:N/A (2023). PubMed: 37833939
- van Bruggen JAC et al. Depletion of CLL cells by venetoclax treatment reverses oxidative stress and impaired glycolysis in CD4 T cells. Blood Adv 6:4185-4195 (2022). PubMed: 35580333
- Kristiansen CK et al. Comparing the mitochondrial signatures in ESCs and iPSCs and their neural derivations. Cell Cycle 21:2206-2221 (2022). PubMed: 35815665
- Wu P et al. Ginsenoside Rg3 alleviates septic liver injury by regulating the lncRNA TUG1/miR-200c-3p/SIRT1 axis. J Inflamm (Lond) 18:31 (2021). PubMed: 34930287
- Rodríguez-Pascau L et al. PPAR gamma agonist leriglitazone improves frataxin-loss impairments in cellular and animal models of Friedreich Ataxia. Neurobiol Dis 148:105162 (2021). PubMed: 33171227
