为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Signal Transduction Metabolism Energy Metabolism

Anti-PGAM1抗体(ab2220)

  • Datasheet
Reviews (1)Q&A (1)References (7)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-PGAM1 antibody (ab2220)
  • Western blot - Anti-PGAM1 antibody (ab2220)

Key features and details

  • Goat polyclonal to PGAM1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

你可能会感兴趣

蛋白
Product image
Recombinant Human PGAM1 protein (ab100885)
一抗
Product image
Anti-TMPRSS4 antibody (ab82176)
二抗
Product image
Donkey Anti-Goat IgG H&L (HRP) (ab97110)

查看更多相关产品

概述

  • 产品名称

    Anti-PGAM1抗体
    参阅全部 PGAM1 一抗
  • 描述

    山羊多克隆抗体to PGAM1
  • 宿主

    Goat
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human PGAM1 aa 200 to the C-terminus (C terminal). (NP_000281.2; NP_001025062.1)
    Database link: NP_002620.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of carbohydrates
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Carbohydrate metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human PGAM1 protein (ab100885)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2220于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.

1 hour primary incubation is recommended for this product.

说明
WB
Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 28 kDa.

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • 组织特异性

    In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • 序列相似性

    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Target information above from: UniProt accession P18669 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 404148 Cow
    • Entrez Gene: 5223 Human
    • Entrez Gene: 18648 Mouse
    • Entrez Gene: 24642 Rat
    • Omim: 172250 Human
    • SwissProt: Q3SZ62 Cow
    • SwissProt: P18669 Human
    • SwissProt: Q9DBJ1 Mouse
    • SwissProt: P25113 Rat
    • Unigene: 592599 Human
    • Unigene: 632918 Human
    • Unigene: 391589 Mouse
    • Unigene: 480556 Mouse
    • Unigene: 1383 Rat
    • Unigene: 154337 Rat
    see all
  • 别名

    • BPG dependent PGAM 1 antibody
    • BPG dependent PGAM1 antibody
    • BPG-dependent PGAM 1 antibody
    • OTTHUMP00000059414 antibody
    • PGAM 1 antibody
    • PGAM A antibody
    • PGAM B antibody
    • PGAM-B antibody
    • PGAM1 antibody
    • PGAM1_HUMAN antibody
    • PGAMA antibody
    • PGAMB antibody
    • Phosphoglycerate mutase 1 antibody
    • Phosphoglycerate mutase 1 brain antibody
    • Phosphoglycerate mutase A antibody
    • Phosphoglycerate mutase A nonmuscle form antibody
    • Phosphoglycerate mutase isozyme B antibody
    see all

图片

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    Anti-PGAM1 antibody (ab2220) at 0.01 µg/ml + Human liver lysate at 35 µg

    Developed using the ECL technique.


    Primary incubated for 1 hour

  • Western blot - Anti-PGAM1 antibody (ab2220)
    Western blot - Anti-PGAM1 antibody (ab2220)
    ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. ab2220 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (7)

发表研究结果有使用 ab2220?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2220 被引用在 7 文献中.

  • Huang K  et al. A Novel Allosteric Inhibitor of Phosphoglycerate Mutase 1 Suppresses Growth and Metastasis of Non-Small-Cell Lung Cancer. Cell Metab 30:1107-1119.e8 (2019). PubMed: 31607564
  • Okuda J  et al. Persistent overexpression of phosphoglycerate mutase, a glycolytic enzyme, modifies energy metabolism and reduces stress resistance of heart in mice. PLoS One 8:e72173 (2013). WB ; Mouse . PubMed: 23951293
  • Hallows WC  et al. Regulation of Glycolytic Enzyme Phosphoglycerate Mutase-1 by Sirt1 Protein-mediated Deacetylation. J Biol Chem 287:3850-8 (2012). WB ; Human . PubMed: 22157007
  • Lei Y  et al. Proteomics Identification of ITGB3 as a Key Regulator in Reactive Oxygen Species-induced Migration and Invasion of Colorectal Cancer Cells. Mol Cell Proteomics 10:M110.005397 (2011). WB, IHC-P ; Human . PubMed: 21622897
  • Klein-Scory S  et al. Immunoscreening of the extracellular proteome of colorectal cancer cells. BMC Cancer 10:70 (2010). WB ; Human . PubMed: 20184735
  • Evans MJ  et al. Mechanistic and structural requirements for active site labeling of phosphoglycerate mutase by spiroepoxides. Mol Biosyst 3:495-506 (2007). PubMed: 17579775
  • Evans MJ  et al. Target discovery in small-molecule cell-based screens by in situ proteome reactivity profiling. Nat Biotechnol 23:1303-7 (2005). PubMed: 16200062

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-PGAM1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (BRAIN)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
BRAIN
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 16 2015

Question

I wonder about specificity of that antibody. Does it really recognize only PGAM1 and not PGAM2 form?

Read More

Abcam community

Verified customer

Asked on Nov 06 2007

Answer

Thank you for your enquiry. I have blasted the immunizing peptide sequence and found that 13 out of 14 amino acids were identical to the sequence of PGAM2. Therefore, it is likely that this product might cross react with PGAM2. However, since we have not tested it in our laboratory, we are not sure of the results. I hope this information will be helpful. Should you require anything else, please do not hesitate to contact me.

Read More

Abcam Scientific Support

回复于 Nov 07 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有