Anti-PFTK1抗体(ab224098)
Key features and details
- Rabbit polyclonal to PFTK1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PFTK1抗体 -
描述
兔多克隆抗体to PFTK1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Common marmoset, Xenopus tropicalis, Nine-banded armadillo, Titi
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免疫原
Recombinant fragment corresponding to Human PFTK1 aa 1-200.
Database link: O94921 -
阳性对照
- IHC-P: Human cerebral cortex, testis, kidney tissues. ICC/IF: U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224098于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport. -
组织特异性
Highly expressed in brain, pancreas, kidney, heart, testis and ovary. Also detected at lower levels in other tissues except in spleen and thymus where expression is barely detected. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cell membrane. Cytoplasm. Nucleus. Recruited to the cell membrane by CCNY. - Information by UniProt
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数据库链接
- Entrez Gene: 5218 Human
- Entrez Gene: 18647 Mouse
- Entrez Gene: 100328634 Rabbit
- Omim: 610679 Human
- SwissProt: O94921 Human
- SwissProt: O35495 Mouse
- SwissProt: B6A7Q3 Rabbit
- Unigene: 258576 Human
see all -
别名
- cdk14 antibody
- CDK14_HUMAN antibody
- Cell division protein kinase 14 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (ab224098)
Immunohistochemical analysis of human kidney labeling PFTK1 with ab224098 at a 1/20 dilution. Moderate cytoplasmic positivity in cells in tubules.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (ab224098)Immunohistochemical analysis of human testis labeling PFTK1 with ab224098 at a 1/20 dilution. Moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFTK1 antibody (ab224098)Paraffin-embedded human cerebral cortex tissue stained for PFTK1 using ab224098 at 1/20 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PFTK1 antibody (ab224098)PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for PFTK1 (green) using ab224098 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab224098 被引用在 3 文献中.
- Du C et al. Hsa_circRNA_102229 facilitates the progression of triple-negative breast cancer via regulating the miR-152-3p/PFTK1 pathway. J Gene Med 23:e3365 (2021). PubMed: 34031947
- Zhao X et al. Cytoplasmic Localization Isoform of Cyclin Y Enhanced the Metastatic Ability of Lung Cancer via Regulating Tropomyosin 4. Front Cell Dev Biol 9:684819 (2021). PubMed: 34222253
- Li SM et al. The putative tumour suppressor miR-1-3p modulates prostate cancer cell aggressiveness by repressing E2F5 and PFTK1. J Exp Clin Cancer Res 37:219 (2018). PubMed: 30185212