Anti-PFKP抗体[OTI1D6] (ab119796)

Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am sending you our WB questionnaire as I would like to focus on one application first. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Order Details Antibody code: ab119796 Anti-PFKP antibody [1D6]
Problem Choose: Non-specific band Multiple bands No signal or weak signal High background
Lot number
Purchase order number or preferably Abcam order number:
General Information Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, wrong band size, more bands, no band etc.)
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
Amount of protein loaded
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Detection method (ECL, ECLPlus etc.)
Positive and negative controls used (please specify)
Optimization attempts (problem solving) How many times have you tried the Western?
Have you run a "No Primary" control? Yes No
Do you obtain the same results every time? Yes No e.g. are the background bands always in the same place?
What steps have you altered?
Additional Notes:
Image: We would appreciate if you are able to provide an image (including molecular weight markers) which would help us to asses the results.