重组Anti-PFKFB4抗体[EPR28633-53] (ab318193)
![Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)](https://www.abcam.cn/ps/products/318/ab318193/Images/ab318193-542834-anti-pfkfb4antibody-epr28633-53-western-blot-hela-human-cervical-adenocarcinoma-epithelial-cell-transfected-with-scrambled-sirna-control-whole-cell-lysate-50-ug.jpg "Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28633-53] to PFKFB4
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PFKFB4抗体[EPR28633-53]
参阅全部 PFKFB4 一抗 -
描述
兔单克隆抗体[EPR28633-53] to PFKFB4 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa transfected with scrambled siRNA control, Raji, PC-3 and 293T cells transfected with a Human PFKFB4 expression vector containing a His-tag, whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28633-53 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab318193于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 54 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 54 kDa. |
靶标
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功能
Synthesis and degradation of fructose 2,6-bisphosphate. -
序列相似性
In the C-terminal section; belongs to the phosphoglycerate mutase family. - Information by UniProt
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数据库链接
- Entrez Gene: 5207 Human
- Entrez Gene: 5208 Human
- Entrez Gene: 5209 Human
- Entrez Gene: 5210 Human
- Omim: 605320 Human
- SwissProt: O60825 Human
- SwissProt: P16118 Human
- SwissProt: Q16875 Human
see all -
别名
- 6-bisphosphatase antibody
- 6-P2ase 4 antibody
- 6-P2ASE testis-type isozyme antibody
see all
图片
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Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)All lanes : Anti-PFKFB4 antibody [EPR28633-53] (ab318193) at 1/1000 dilution
Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate
Lane 2 : HeLa transfected with siRNA specifically targeting PFKFB4 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 81 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)](https://www.abcam.cn/ps/products/318/ab318193/Images/ab318193-542835-anti-pfkfb4antibody-epr28633-53-western-blot-raji-human-burkitts-lymphoma-b-lymphocyte-whole-cell-lysate-50-ug.jpg)
Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)All lanes : Anti-PFKFB4 antibody [EPR28633-53] (ab318193) at 1/1000 dilution
Lane 1 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: A549
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)](https://www.abcam.cn/ps/products/318/ab318193/Images/ab318193-542836-anti-pfkfb4antibody-epr28633-53-western-blot-293t-cells-transfected-with-a-human-pfkfb1-expression-vector-containing-a-his-tag-whole-cell-lysate-4-ug.jpg)
Western blot - Anti-PFKFB4 antibody [EPR28633-53] (ab318193)All lanes : Anti-PFKFB4 antibody [EPR28633-53] (ab318193) at 1/1000 dilution
Lane 1 : 293T cells transfected with a Human PFKFB1 expression vector containing a His-tag, whole cell lysate
Lane 2 : 293T cells transfected with a Human PFKFB2 expression vector containing a His-tag, whole cell lysate
Lane 3 : 293T cells transfected with a Human PFKFB3 expression vector containing a His-tag, whole cell lysate
Lane 4 : 293T cells transfected with a Human PFKFB4 expression vector containing a His-tag, whole cell lysate
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human PFKFB1, PFKFB2, PFKFB3.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
In Western blot, Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) staining at 1/5000 dilution.
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![Anti-PFKFB4 antibody [EPR28633-53] (ab318193)](https://www.abcam.cn/ps/products/318/ab318193/Images/ab318193-4-benefitsofrecombinantantibodies.jpg)
Anti-PFKFB4 antibody [EPR28633-53] (ab318193)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab318193 尚未被引用在任何文献中。