重组Anti-PFDN5抗体[EPR7755] - BSA and Azide free (ab248313)
![Western blot - Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)](https://www.abcam.cn/ps/products/248/ab248313/Images/ab248313-402384-anti-pfdn5-antibody-epr7755-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7755] to PFDN5 - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-PFDN5抗体[EPR7755] - BSA and Azide free
参阅全部 PFDN5 一抗 -
描述
兔单克隆抗体[EPR7755] to PFDN5 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
ab248313 is the carrier-free version of ab129116.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7755 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab248313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa. |
靶标
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功能
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. -
组织特异性
Highly expressed in pancreas and skeletal muscle and moderately in other tissues. -
序列相似性
Belongs to the prefoldin subunit alpha family. - Information by UniProt
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数据库链接
- Entrez Gene: 5204 Human
- Entrez Gene: 56612 Mouse
- Entrez Gene: 300257 Rat
- Omim: 604899 Human
- SwissProt: Q99471 Human
- SwissProt: Q9WU28 Mouse
- Unigene: 655327 Human
- Unigene: 158264 Mouse
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别名
- 1190001O17Rik antibody
- 1700010A06Rik antibody
- c myc binding protein antibody
see all
图片
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Western blot - Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)All lanes : Anti-PFDN5 antibody [EPR7755] (ab129116) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal brain lysate
Lane 4 : Fetal heart lysate
Lane 5 : Fetal kidney lysate
Lane 6 : Human spleen lysate
Lane 7 : HepG2 cell lysate
Lane 8 : NIH/3T3 lysate
Lane 9 : F9 cell lysate
Lane 10 : Fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDaThis data was developed using ab129116, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)](https://www.abcam.cn/ps/products/248/ab248313/Images/ab248313-1-anti-pfdn5-antibody-epr7755-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)This data was developed using ab129116, the same antibody clone in a different buffer formulation.ab129116, at a 1/100 dilution, staining PFDN5 in paraffin-embedded Human colon carcinoma tissue by immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. -
![Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)](https://www.abcam.cn/ps/products/248/ab248313/Images/ab248313-3-benefits-of-recombinant-antibodies.png)
Anti-PFDN5 antibody [EPR7755] - BSA and Azide free (ab248313)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab248313 尚未被引用在任何文献中。
