重组Anti-PF4抗体[EPR7763] (ab129183)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-143216-anti-pf4-antibody-epr7763-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7763] to PF4
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-PF4抗体[EPR7763]
参阅全部 PF4 一抗 -
描述
兔单克隆抗体[EPR7763] to PF4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human
预测可用于: Rat
-
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- Human spleen and platelet lysates, Human spleen tissue
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
解离常数（KD）
KD = 5.40 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7763 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129183于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 11 kDa.
|
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 11 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
序列相似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻译后修饰
Binds non-covalently to a proteoglycan molecule. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 5196 Human
- Entrez Gene: 360918 Rat
- Omim: 173460 Human
- SwissProt: P02776 Human
- SwissProt: P06765 Rat
- Unigene: 81564 Human
-
别名
- C-X-C motif chemokine 4 antibody
- Chemokine (C X C motif) ligand 4 antibody
- Chemokine (CXC motif) ligand 4 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)
ab129183, at a 1/100 dilution, staining PF4 in paraffin embedded Human spleen tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-487461-ab129183IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)Immunohistochemical analysis of parafifin-embedded sections of human spleen tissue with primary antibody ab129183 at 1/200 dilution and incubated at +4°C overnight, followed by secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Platelet staining on human spleen is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (citrate buffer, pH 6.0) ab93678.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-487464-ab129183IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)Immunohistochemical analysis of parafifin-embedded sections of mouse liver tissue with primary antibody ab129183 at 1/200 dilution and incubated at +4°C overnight, followed by secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Sporadically platelet staining on mouse liver is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (citrate buffer, pH 6.0) ab93678.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-487465-ab129183IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PF4 antibody [EPR7763] (ab129183)Immunohistochemical analysis of parafifin-embedded sections of rat liver tissue with primary antibody ab129183 at 1/200 dilution and incubated at +4°C overnight, followed by secondary antibody Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on rat liver is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (citrate buffer, pH 6.0) ab93678.
-
![Western blot - Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-143217-anti-pf4-antibody-epr7763-western-blot.jpg)
Western blot - Anti-PF4 antibody [EPR7763] (ab129183)All lanes : Anti-PF4 antibody [EPR7763] (ab129183) at 1/1000 dilution
Lane 1 : Human spleen lysates
Lane 2 : Human platelet lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 11 kDa -
![OI-RD Scanning - Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-143215-anti-pf4-antibody-epr7763.jpg)
OI-RD Scanning - Anti-PF4 antibody [EPR7763] (ab129183) -
![Anti-PF4 antibody [EPR7763] (ab129183)](https://www.abcam.cn/ps/products/129/ab129183/Images/ab129183-4-benefits-of-recombinant-antibodies.png)
Anti-PF4 antibody [EPR7763] (ab129183)
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab129183 被引用在 5 文献中.
- Kriek N et al. Cucurbitacins Elicit Anti-Platelet Activity via Perturbation of the Cytoskeleton and Integrin Function. Thromb Haemost 122:1115-1129 (2022). PubMed: 35253142
- Zhang X et al. Comparison of serum and plasma as a source of blood extracellular vesicles: Increased levels of platelet-derived particles in serum extracellular vesicle fractions alter content profiles from plasma extracellular vesicle fractions. PLoS One 17:e0270634 (2022). PubMed: 35749554
- Vijayan K et al. A genome-wide CRISPR-Cas9 screen identifies CENPJ as a host regulator of altered microtubule organization during Plasmodium liver infection. Cell Chem Biol 29:1419-1433.e5 (2022). PubMed: 35738280
- Chacón-Fernández P et al. Brain-derived Neurotrophic Factor in Megakaryocytes. J Biol Chem 291:9872-81 (2016). PubMed: 27006395
- Li J et al. CXCL4 Contributes to the Pathogenesis of Chronic Liver Allograft Dysfunction. J Immunol Res 2016:9276986 (2016). IHC . PubMed: 28053995