Anti-PEX14抗体(ab109999)
Key features and details
- Rabbit polyclonal to PEX14
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PEX14抗体
参阅全部 PEX14 一抗 -
描述
兔多克隆抗体to PEX14 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
A synthesized peptide derived from internal of Human PEX14.
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阳性对照
- Human breast carcinoma tissue A549 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Frozen Stock (-20C). Shelf life 12 months. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109999于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 41 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 41 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Component of the peroxisomal translocation machinery with PEX13 and PEX17. Interacts with both the PTS1 and PTS2 receptors. Binds directly to PEX17. -
疾病相关
Defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK) [MIM:601791]. PBD-CGK is a peroxisomal disorder arising from a failure of protein import into the peroxisomal membrane or matrix. The peroxisome biogenesis disorders (PBD group) are genetically heterogeneous with at least 14 distinct genetic groups as concluded from complementation studies. Include disorders are: Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), and classical rhizomelic chondrodysplasia punctata (RCDP). ZWS, NALD and IRD are distinct from RCDP and constitute a clinical continuum of overlapping phenotypes known as the Zellweger spectrum (PBD-ZSS).
Defects in PEX14 are a cause of Zellweger syndrome (ZWS) [MIM:214100]. ZWS is a fatal peroxisome biogenesis disorder characterized by dysmorphic facial features, hepatomegaly, ocular abnormalities, renal cysts, hearing impairment, profound psychomotor retardation, severe hypotonia and neonatal seizures. Death occurs within the first year of life. -
序列相似性
Belongs to the peroxin-14 family. -
细胞定位
Peroxisome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5195 Human
- Entrez Gene: 56273 Mouse
- Entrez Gene: 64460 Rat
- Omim: 601791 Human
- SwissProt: O75381 Human
- SwissProt: Q9R0A0 Mouse
- SwissProt: Q642G4 Rat
- Unigene: 149983 Human
see all -
别名
- dJ734G22.2 antibody
- NAPP2 antibody
- NF E2 associated polypeptide 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (ab109999)Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab109999.
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Western blot - Anti-PEX14 antibody (ab109999)All lanes : Anti-PEX14 antibody (ab109999) at 1/500 dilution
Lane 1 : A549 cells
Lane 2 : A549 cells treated with the synthesized peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 41 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab109999 被引用在 3 文献中.
- Xia Q et al. S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1. Cell Death Dis 9:657 (2018). PubMed: 29844306
- Wang W et al. TRIM37, a novel E3 ligase for PEX5-mediated peroxisomal matrix protein import. J Cell Biol 216:2843-2858 (2017). PubMed: 28724525
- Zhang J et al. ATM functions at the peroxisome to induce pexophagy in response to ROS. Nat Cell Biol 17:1259-1269 (2015). WB ; Human . PubMed: 26344566