AB109999

Anti-PEX14抗体

Name: PEX14抗体 (ab109999)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109999
Price: 4606 CNY
Availability: InStock

Anti-PEX14 antibody

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(3 Publications)

Rabbit Polyclonal PEX14 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PEX14.

查看别名

Peroxisomal membrane protein PEX14, PTS1 receptor-docking protein, Peroxin-14, Peroxisomal membrane anchor protein PEX14, PEX14

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (AB109999)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (AB109999)

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using ab109999.

Unknown

Western blot - Anti-PEX14 antibody (AB109999)

All lanes:

Western blot - Anti-PEX14 antibody (ab109999) at 1/500 dilution

Lane 1:

A549 cells at 5 µg

Lane 2:

A549 cells treated with the synthesized peptide at 5 µg

Predicted band size: 15 kDa,23 kDa,41 kDa,59 kDa,73 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PEX14. The exact immunogen used to generate this antibody is proprietary information.

O75381

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PEX14 also known as Peroxisomal Membrane Protein 14 is an essential component of the peroxisomal membrane acting as an integral membrane protein. PEX14 has a mass of approximately 47 kDa and is expressed in many tissues including liver and kidney. It functions mechanically as a docking factor within the peroxisomal matrix enabling the import of proteins into peroxisomes which are cellular organelles responsible for various metabolic functions.

Biological function summary

PEX14 plays a significant role in maintaining peroxisome structure and function. It is part of the peroxisome importomer complex which facilitates the import of matrix proteins by interacting with peroxins such as PEX5. PEX14's interaction with these proteins ensures the proper translocation and sorting of enzymes critical for peroxisomal activities like fatty acid oxidation and reactive oxygen species detoxification.

Pathways

PEX14 is involved in key metabolic and cellular pathways including peroxisomal fatty acid beta-oxidation and ether lipid metabolism. It directly associates with PEX5 a peroxin that transports cargo proteins. In these pathways PEX14 plays a central role in maintaining metabolic processes that are essential for lipid metabolism and cellular detoxification.

PEX14’s malfunction or absence can contribute to Peroxisome Biogenesis Disorders (PBDs) such as Zellweger syndrome. These disorders significantly affect cellular metabolism and result in severe developmental issues. PEX14's interactions with proteins like PEX19 affect peroxisomal membrane biogenesis further linking PEX14 to these metabolic disorders and highlighting its importance in peroxisomal function.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Component of the PEX13-PEX14 docking complex, a translocon channel that specifically mediates the import of peroxisomal cargo proteins bound to PEX5 receptor (PubMed : 24235149, PubMed : 28765278, PubMed : 9653144). The PEX13-PEX14 docking complex forms a large import pore which can be opened to a diameter of about 9 nm (By similarity). Mechanistically, PEX5 receptor along with cargo proteins associates with the PEX14 subunit of the PEX13-PEX14 docking complex in the cytosol, leading to the insertion of the receptor into the organelle membrane with the concomitant translocation of the cargo into the peroxisome matrix (PubMed : 24235149, PubMed : 28765278). Plays a key role for peroxisome movement through a direct interaction with tubulin (PubMed : 21525035).

See full target information PEX14

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 9:657 PubMed29844306

2018

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Xia,Xing Li,Huijuan Zhou,Lu Zheng,Jing Shi

The Journal of cell biology 216:2843-2858 PubMed28724525

2017

TRIM37, a novel E3 ligase for PEX5-mediated peroxisomal matrix protein import.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Zhi-Jie Xia,Jean-Claude Farré,Suresh Subramani

Nature cell biology 17:1259-1269 PubMed26344566

2015

ATM functions at the peroxisome to induce pexophagy in response to ROS.

Applications

Species

Human

Jiangwei Zhang,Durga Nand Tripathi,Ji Jing,Angela Alexander,Jinhee Kim,Reid T Powell,Ruhee Dere,Jacqueline Tait-Mulder,Ji-Hoon Lee,Tanya T Paull,Raj K Pandita,Vijaya K Charaka,Tej K Pandita,Michael B Kastan,Cheryl Lyn Walker

View all publications

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Anti-PEX14抗体

Anti-PEX14 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PEX14 antibody (AB109999)

Western blot - Anti-PEX14 antibody (AB109999)