Anti-Pescadillo抗体(ab72539)
Key features and details
- Rabbit polyclonal to Pescadillo
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Pescadillo抗体
参阅全部 Pescadillo 一抗 -
描述
兔多克隆抗体to Pescadillo -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residue 535 and the C-terminus (residue 588) of human Pescadillo (NP_055118.1)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72539于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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WB
Use a concentration of 0.1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. -
组织特异性
Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. -
序列相似性
Belongs to the pescadillo family.
Contains 1 BRCT domain. -
翻译后修饰
Sumoylated. -
细胞定位
Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. - Information by UniProt
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数据库链接
- Entrez Gene: 23481 Human
- Omim: 605819 Human
- SwissProt: O00541 Human
- Unigene: 517543 Human
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别名
- FLJ43790 antibody
- Homo sapiens pescadillo mRNA complete cds antibody
- PES antibody
see all
图片
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Western blot - Anti-Pescadillo antibody (ab72539)All lanes : Anti-Pescadillo antibody (ab72539) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 68 kDa -
Immunoprecipitation - Anti-Pescadillo antibody (ab72539)Detection of Human Pescadillo by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72539 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab72539 被引用在 5 文献中.
- Xu KD et al. Licochalcone A inhibits cell growth through the downregulation of the Hippo pathway via PES1 in cholangiocarcinoma cells. Environ Toxicol 37:564-573 (2022). PubMed: 34845814
- Jiang Z et al. microRNA-1271 impedes the development of prostate cancer by downregulating PES1 and upregulating ERß. J Transl Med 18:209 (2020). PubMed: 32448371
- Qiu YB et al. PES1 promotes the occurrence and development of papillary thyroid cancer by upregulating the ERa/ERß protein ratio. Sci Rep 9:1032 (2019). PubMed: 30705367
- Rankin CR et al. Linear and circular CDKN2B-AS1 expression is associated with Inflammatory Bowel Disease and participates in intestinal barrier formation. Life Sci 231:116571 (2019). PubMed: 31207308
- Jin X et al. PES1 promotes BET inhibitors resistance and cells proliferation through increasing c-Myc expression in pancreatic cancer. J Exp Clin Cancer Res 38:463 (2019). PubMed: 31718704