Anti-PERP抗体

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody (AB5986)

Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.

• WB

Unknown

Western blot - Anti-PERP antibody (AB5986)

Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 μg /ml. Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml.

All lanes:

Western blot - Anti-PERP antibody (ab5986)

Predicted band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-PERP antibody (AB5986)

All lanes:

Western blot - Anti-PERP antibody (ab5986) at 1 µg

Lane 1:

293 cell lysate at 15 µg

Lane 2:

A549 cell lysate at 15 µg

Lane 3:

HeLa cell lysate at 15 µg

Lane 4:

HepG2 cell lysate at 15 µg

Lane 5:

K569 cell lysate at 15 µg

Lane 6:

MCF-7 cell lysate at 15 µg

Lane 7:

SK-N-SH cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 21 kDa

false

• WB

CiteAb

Western blot - Anti-PERP antibody (AB5986)

PERP western blot using anti-PERP antibody ab5986. Publication image and figure legend from Marthandan, S., Priebe, S., et al., 2015, Biomed Res Int, PubMed 26339636.

ab5986 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5986 please see the product overview.

Comparison of expression changes between young and old MRC-5 and HFF fibroblasts measured with RNA-seq and Western Blots. (a) 8 genes commonly downregulated and (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue : RNA-seq; red : Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first and the last PD investigated here (RNA-seq : log 2 RPKM FC; protein : log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young and old PD (RNA-seq : DESeq; rRT-PCR/Protein : Student's t-test; n = 3) are indicated with an asterix : *p < 0.05, **p < 0.01, and ***p < 0.001. (c) The blots show the protein expression levels in MRC-5 and HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots.

false