重组Anti-Peroxiredoxin 6抗体[EPR3754] (ab133348)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3754] to Peroxiredoxin 6
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
-
产品名称
Anti-Peroxiredoxin 6抗体[EPR3754]
参阅全部 Peroxiredoxin 6 一抗 -
描述
兔单克隆抗体[EPR3754] to Peroxiredoxin 6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Pig -
免疫原
Synthetic peptide within Human Peroxiredoxin 6 aa 50-150. The exact sequence is proprietary.
-
阳性对照
- HAP1, HeLa, 293 and fetal liver lysates; Human brain tissue
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3754 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 25 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 25 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. -
序列相似性
Belongs to the ahpC/TSA family. Rehydrin subfamily.
Contains 1 thioredoxin domain. -
细胞定位
Cytoplasm. Lysosome. Cytoplasmic vesicle. Also found in lung secretory organelles. - Information by UniProt
-
数据库链接
- Entrez Gene: 9588 Human
- Entrez Gene: 11758 Mouse
- Entrez Gene: 399538 Pig
- Entrez Gene: 94167 Rat
- Omim: 602316 Human
- SwissProt: P30041 Human
- SwissProt: O08709 Mouse
- SwissProt: Q9TSX9 Pig
see all -
别名
- 1 Cys antibody
- 1 Cys peroxiredoxin antibody
- 1 Cys PRX antibody
see all
图片
-
All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : PRDX6 (Peroxiredoxin 6) knockout HAP1 whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : PC3 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDaLanes 1 - 4: Merged signal (red and green). Green - ab133348 observed at 25 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab133348 was shown to specifically react with Peroxiredoxin 6 in wild-type HAP1 cells as signal was lost in PRDX6 (Peroxiredoxin 6) knockout cells. Wild-type and PRDX6 (Peroxiredoxin 6) knockout samples were subjected to SDS-PAGE. Ab133348 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution
Lane 1 : Mouse liver whole cell lysate
Lane 2 : Mouse testis whole cell lysate
Lane 3 : Mouse spleen whole cell lysate
Lane 4 : Rat liver whole cell lysate
Lane 5 : Rat testis whole cell lysate
Lane 6 : Rat spleen whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 15 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
-
All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348) at 1/1000 dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : PRDX6 knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 25 kDaab133348 was shown to react with PRDX6 in wild-type HAP1 cells in Western blot with loss of signal observed in a PRDX6 knockout cell line. Wild-type HAP1 and PRDX6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133348 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
-
All lanes : Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Lane 1 : HeLa lysate
Lane 2 : 293 lysate
Lane 3 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 25 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Peroxiredoxin 6 with ab133348 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Immunohistochemical analysis of paraffin embedded normal Human placenta tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 6 antibody [EPR3754] (ab133348)
Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133348 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
数据表及文件
-
SDS download
-
Datasheet download
文献 (11)
ab133348 被引用在 11 文献中.
- Xia Q et al. Peroxiredoxin 2 is required for the redox mediated adaptation to exercise. Redox Biol 60:102631 (2023). PubMed: 36791646
- Li P et al. Alteration of chromatin high-order conformation associated with oxaliplatin resistance acquisition in colorectal cancer cells. Exploration (Beijing) 3:20220136 (2023). PubMed: 37933235
- Li HT et al. Peroxiredoxin 6 mediates the protective function of curcumin pretreatment in acute lung injury induced by serum from patients undergoing one-lung ventilation in vitro. BMC Pulm Med 22:192 (2022). PubMed: 35549905
- López-Grueso MJ et al. Knockout of PRDX6 induces mitochondrial dysfunction and cell cycle arrest at G2/M in HepG2 hepatocarcinoma cells. Redox Biol 37:101737 (2020). PubMed: 33035814
- Scalabrin M et al. Redox responses in skeletal muscle following denervation. Redox Biol 26:101294 (2019). PubMed: 31450104