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使用敲除细胞株进行验证重组RabMAb

重组Anti-Peroxiredoxin 5抗体[EPR14529(B)] (ab180587)

Submit a review Submit a question References (7)
Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
  • Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14529(B)] to Peroxiredoxin 5
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-Peroxiredoxin 5抗体[EPR14529(B)]
    参阅全部 Peroxiredoxin 5 一抗

  • 描述

    兔单克隆抗体[EPR14529(B)] to Peroxiredoxin 5

  • 宿主

    Rabbit

  • 特异性

    The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 经测试应用

    适用于: WB, IHC-P, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa, HepG2 and A549 cell line lysates; Human kidney tissue; Jurkat cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR14529(B)

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180587于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols
IP
1/50.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 22 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

The mouse and  rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

See IHC antigen retrieval protocols
IP
1/50.

靶标

  • 功能

    Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.

  • 组织特异性

    Widely expressed.

  • 序列相似性

    Belongs to the peroxiredoxin 2 family.
    Contains 1 thioredoxin domain.

  • 细胞定位

    Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • ACR1 antibody
    • Alu co repressor 1 antibody
    • Alu corepressor 1 antibody
    • Antioxidant enzyme B166 antibody
    • AOEB166 antibody
    • B166 antibody
    • epididymis secretory protein Li 55 antibody
    • HEL-S-55 antibody
    • Liver tissue 2D-page spot 71B antibody
    • mitochondrial antibody
    • Peroxiredoxin 5, mitochondrial antibody
    • Peroxiredoxin V antibody
    • Peroxiredoxin-5 antibody
    • Peroxisomal antioxidant enzyme antibody
    • PLP antibody
    • PMP20 antibody
    • PRDX 5 antibody
    • PRDX5 antibody
    • PRDX5_HUMAN antibody
    • PRDX6 antibody
    • Prx-V antibody
    • PRXV antibody
    • SBBI10 antibody
    • Thioredoxin peroxidase PMP20 antibody
    • Thioredoxin reductase antibody
    • TPx type VI antibody
    see all

图片

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: PRDX5 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: A549 whole cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab180587 observed at 17 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab180587 was shown to specifically react with PRDX5 when PRDX5 knockout samples were used. Wild-type and PRDX5 knockout samples were subjected to SDS-PAGE. Ab180587 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling Peroxiredoxin 5 with purified ab180587 at 1:500 dilution (1.18 µg/ml). Heat mediated antigen retrieval was performed using Bond ™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    ab180587 (Purified) at 1:30 dilution (2 µg) immunoprecipitating Peroxiredoxin 5 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
    Lane 2 (+): ab180587 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab180587 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/10000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Human brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution (Purified) + Mouse brain lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunoprecipitation - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunoprecipitation analysis of Peroxiredoxin 5 pulled down from HeLa cell lysates using ab180587 at 1/50 dilution.



    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/1000 dilution (Unpurified) + HeLa cell immunoprecipitated with ab180587 at 1/50.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Observed band size: 17 kDa why is the actual band size different from the predicted?
    Additional bands at: 50 kDa (possible IgG)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling Peroxiredoxin 5 with unpurified ab180587 at 1/250.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    All lanes : Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/5000 dilution (Unpurified)

    Lane 1 : HepG2 cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : A549 cell line lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Western blot - Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587) at 1/20000 dilution (Unpurified) + HeLa cell line lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 17 kDa why is the actual band size different from the predicted?

  • Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)
    Anti-Peroxiredoxin 5 antibody [EPR14529(B)] (ab180587)

文献 (7)

发表研究结果有使用 ab180587？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab180587 被引用在 7 文献中.

  • Liu LW  et al. Gut microbiota-derived nicotinamide mononucleotide alleviates acute pancreatitis by activating pancreatic SIRT3 signalling. Br J Pharmacol 180:647-666 (2023). PubMed: 36321732
  • Queiroz RF  et al. Hydrogen peroxide signaling via its transformation to a stereospecific alkyl hydroperoxide that escapes reductive inactivation. Nat Commun 12:6626 (2021). PubMed: 34785665
  • Cao X  et al. ROS-mediated hypomethylation of PRDX5 promotes STAT3 binding and activates the Nrf2 signaling pathway in NSCLC. Int J Mol Med 47:573-582 (2021). PubMed: 33416106
  • Gao J  et al. Metformin protects against PM2.5-induced lung injury and cardiac dysfunction independent of AMP-activated protein kinase a2. Redox Biol 28:101345 (2020). PubMed: 31669973
  • Baković J  et al. A key metabolic integrator, coenzyme A, modulates the activity of peroxiredoxin 5 via covalent modification. Mol Cell Biochem 461:91-102 (2019). PubMed: 31375973
  • Brown LA  et al. Late life maintenance and enhancement of functional exercise capacity in low and high responding rats after low intensity treadmill training. Exp Gerontol 125:110657 (2019). PubMed: 31306740
  • Wang H  et al. AMPKa2 deficiency exacerbates long-term PM2.5 exposure-induced lung injury and cardiac dysfunction. Free Radic Biol Med 121:202-214 (2018). PubMed: 29753072

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