重组Anti-Peroxiredoxin 3/PRDX3抗体[EPR8114] (ab129206)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8114] to Peroxiredoxin 3/PRDX3
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Peroxiredoxin 3/PRDX3抗体[EPR8114]
参阅全部 Peroxiredoxin 3/PRDX3 一抗 -
描述
兔单克隆抗体[EPR8114] to Peroxiredoxin 3/PRDX3 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
不适用于: IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Peroxiredoxin 3/PRDX3 aa 50-150. The exact sequence is proprietary.
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阳性对照
- HeLa, 293T, MCF-7, and A431 cell lysates; Human liver and thyroid gland carcinoma tissues; MCF-7 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8114 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129206于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/50000 - 1/200000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
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ICC/IF |
1/50 - 1/100.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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说明 |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/50000 - 1/200000. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa). |
ICC/IF
1/50 - 1/100. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
靶标
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功能
Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. -
序列相似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
细胞定位
Mitochondrion. - Information by UniProt
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数据库链接
- Entrez Gene: 10935 Human
- Omim: 604769 Human
- SwissProt: P30048 Human
- Unigene: 523302 Human
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别名
- Antioxidant protein 1 antibody
- AOP 1 antibody
- AOP-1 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 3/PRDX3 knockout HAP1 cell lysate (20 µg)
Lane 3: MCF7 cell lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab129206 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab129206 was shown to specifically react with Peroxiredoxin 3/PRDX3 when Peroxiredoxin 3/PRDX3 knockout samples were used. Wild-type and Peroxiredoxin 3/PRDX3 knockout samples were subjected to SDS-PAGE. ab129206 and ab8245 (loading control to GAPDH) were both diluted 1/50000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in MCF-7 cells by Immunofluorescence.
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Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling Peroxiredoxin 3/PRDX3 with unpurified ab129206 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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All lanes : Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 3/PRDX3 antibody [EPR8114] (ab129206)
ab129206, at 1/50 dilution, staining Peroxiredoxin 3/PRDX3 in paraffin-embedded Human thyroid gland carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab129206 被引用在 2 文献中.
- Yeung ATY et al. A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Salmonella Infection. mBio 10:N/A (2019). PubMed: 31594818
- Powers JF et al. A unique model for SDH-deficient GIST: an endocrine-related cancer. Endocr Relat Cancer 25:943-954 (2018). PubMed: 29967109