Anti-Peroxiredoxin 2/PRP抗体(ab226922)
Key features and details
- Rabbit polyclonal to Peroxiredoxin 2/PRP
- Suitable for: WB, IHC-Fr, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Peroxiredoxin 2/PRP抗体
参阅全部 Peroxiredoxin 2/PRP 一抗 -
描述
兔多克隆抗体to Peroxiredoxin 2/PRP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, IHC-Pmore details -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Sheep, Cow, Pig, Rhesus monkey
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免疫原
Recombinant fragment within Human Peroxiredoxin 2/PRP (internal sequence). The exact sequence is proprietary.
Database link: P32119 -
阳性对照
- WB: A549 and HCT 116 whole cell extracts. IHC-P: Human breast cancer tissue. IHC-Fr: Rat liver tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226922于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 16,22 kDa.
|
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| IHC-Fr |
1/100 - 1/1000.
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| IHC-P |
1/100 - 1/1000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 16,22 kDa. |
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IHC-Fr
1/100 - 1/1000. |
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IHC-P
1/100 - 1/1000. |
靶标
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功能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). -
序列相似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 286793 Cow
- Entrez Gene: 7001 Human
- Entrez Gene: 21672 Mouse
- Entrez Gene: 29338 Rat
- Omim: 600538 Human
- SwissProt: Q9BGI3 Cow
- SwissProt: P32119 Human
- SwissProt: Q61171 Mouse
see all -
别名
- Epididymis secretory sperm binding protein Li 2a antibody
- HEL S 2a antibody
- MGC4104 antibody
see all
图片
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Western blot - Anti-Peroxiredoxin 2/PRP antibody (ab226922)All lanes : Anti-Peroxiredoxin 2/PRP antibody (ab226922) at 1/5000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell extract
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell extract
Lane 3 : HCT 116 (human colorectal carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 16,22 kDa15% SDS-PAGE gel.
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Immunohistochemistry (Frozen sections) - Anti-Peroxiredoxin 2/PRP antibody (ab226922)Frozen section of rat liver tissue stained for Peroxiredoxin 2/PRP using ab226922 at 1/200 dilution in immunohistochemical analysis, followed by a fluorescent secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 2/PRP antibody (ab226922)Paraffin-embedded human breast cancer tissue stained for Peroxiredoxin 2/PRP using ab226922 at 1/500 dilution in immunohistochemical analysis.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226922 尚未被引用在任何文献中。