重组Anti-Peroxiredoxin 1/PAG抗体[EPR5433] (ab109498)
![Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)](https://www.abcam.cn/ps/products/109/ab109498/Images/ab109498-260945-anti-peroxiredoxin-1-pag-antibody-epr5433-western-blot.jpg "Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5433] to Peroxiredoxin 1/PAG
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Peroxiredoxin 1/PAG抗体[EPR5433]
参阅全部 Peroxiredoxin 1/PAG 一抗 -
描述
兔单克隆抗体[EPR5433] to Peroxiredoxin 1/PAG -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK293T, Jurkat, HAP1, A431, MCF-7, NIH/3T3, K562 and Mouse brain cell lysates. IHC-P: Kidney tissue and Thyroid carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5433 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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| IHC-P |
1/1000 - 1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval |
| 说明 |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
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IHC-P
1/1000 - 1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval |
靶标
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功能
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. -
序列相似性
Belongs to the ahpC/TSA family.
Contains 1 thioredoxin domain. -
翻译后修饰
Phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity. -
细胞定位
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 5052 Human
- Entrez Gene: 18477 Mouse
- Omim: 176763 Human
- SwissProt: Q06830 Human
- SwissProt: P35700 Mouse
- Unigene: 180909 Human
- Unigene: 30929 Mouse
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别名
- Heme binding 23 kDa protein antibody
- MSP23 antibody
- Natural killer cell-enhancing factor A antibody
see all
图片
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Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109498 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.ab109498 was shown to specifically react with Peroxiredoxin 1/PAG when Peroxiredoxin 1/PAG knockout samples were used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)](https://www.abcam.cn/ps/products/109/ab109498/Images/ab109498-395406-anti-peroxiredoxin-1pag-antibody-epr5433-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)All lanes : Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PRDX1 knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab109498 observed at 26 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109498 Anti-Peroxiredoxin 1/PAG antibody [EPR5433] was shown to specifically react with Peroxiredoxin 1/PAG in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266842 (knockout cell lysate ab257040) was used. Wild-type and Peroxiredoxin 1/PAG knockout samples were subjected to SDS-PAGE. ab109498 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)](https://www.abcam.cn/ps/products/109/ab109498/Images/ab109498-471093-antiperoxiredoxin-1pag-antibody-epr5433-western-blot.jpg)
Western blot - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)All lanes : Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498) at 1/10000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : Mouse brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)](https://www.abcam.cn/ps/products/109/ab109498/Images/ab109498-471094-antiperoxiredoxin-1pag-antibody-epr5433-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling Peroxiredoxin 1/PAG with ab109498 at 1/1000 (0.155 ug/ml) dilution. The section was incubated with ab109498 for 30 mins at room temperature and followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.
Positive staining on the human thyroid cancer, performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)](https://www.abcam.cn/ps/products/109/ab109498/Images/ab109498-471095-antiperoxiredoxin-1pag-antibody-epr5433-immunohistochemistry-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Peroxiredoxin 1/PAG with ab109498 at 1/4000 (0.038 ug/ml) dilution. The section was incubated with ab109498 for 30 mins at room temperature and followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.
Positive staining on the mouse kidney, performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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![Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)](https://www.abcam.cn/ps/products/109/ab109498/Images/ab109498-8-benefits-of-recombinant-antibodies.png)
Anti-Peroxiredoxin 1/PAG antibody [EPR5433] (ab109498)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab109498 被引用在 7 文献中.
- Xia Q et al. Peroxiredoxin 2 is required for the redox mediated adaptation to exercise. Redox Biol 60:102631 (2023). PubMed: 36791646
- Xu S et al. Cullin-5 neddylation-mediated NOXA degradation is enhanced by PRDX1 oligomers in colorectal cancer. Cell Death Dis 12:265 (2021). PubMed: 33712558
- Jiang Y et al. LncRNA LINC00460 promotes EMT in head and neck squamous cell carcinoma by facilitating peroxiredoxin-1 into the nucleus. J Exp Clin Cancer Res 38:365 (2019). PubMed: 31429766
- Shi XJ et al. Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I. Antioxid Redox Signal 27:73-92 (2017). Human . PubMed: 27650197
- Ziegler YS et al. Integration of Breast Cancer Secretomes with Clinical Data Elucidates Potential Serum Markers for Disease Detection, Diagnosis, and Prognosis. PLoS One 11:e0158296 (2016). WB . PubMed: 27355404
- Riquier S et al. Peroxiredoxin post-translational modifications by redox messengers. Redox Biol 2:777-85 (2014). WB ; Mouse . PubMed: 25009779
- Gambari L et al. Sodium hydrosulfide inhibits the differentiation of osteoclast progenitor cells via NRF2-dependent mechanism. Pharmacol Res 87:99-112 (2014). Human . PubMed: 24998607