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Anti-Peripherin抗体[8G2] (ab4653)

Reviews (1)Q&A (1)References (3)
Western blot - Anti-Peripherin antibody [8G2] (ab4653)
  • Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (ab4653)

Key features and details

  • Mouse monoclonal [8G2] to Peripherin
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Peripherin抗体[8G2]
    参阅全部 Peripherin 一抗

  • 描述

    小鼠单克隆抗体[8G2] to Peripherin

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Cow, Human

  • 免疫原

    Recombinant Rat Peripherin purified from E. coli.

  • 阳性对照

    • WB: Mouse, rat, cow spinal cord lysate, SH-SY5Y, PC-12 whole cell lysate. ICC: SH-SY5Y, PC-12 cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4653于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF
1/500.
说明
WB
1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
ICC/IF
1/500.

靶标

图片

  • Western blot - Anti-Peripherin antibody [8G2] (ab4653)
    Western blot - Anti-Peripherin antibody [8G2] (ab4653)
    All lanes : Anti-Peripherin antibody [8G2] (ab4653) at 1/500 dilution

    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Rat spinal cord lysate
    Lane 4 : Cow spinal cord lysate
    Lane 5 : SH-SY5Y (human bone marrow neuroblastoma cell) whole cell lysate
    Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate

    Secondary
    All lanes : Anti-Mouse IgG

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 25 kDa (possible IgG), 50 kDa (possible IgG)



    The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.

  • Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (ab4653)
    Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (ab4653)

    Immunofluorescent analysis of SH-SY5Y (human bone marrow neuroblastoma cell) cells (A) and PC-12 (rat adrenal gland pheochromocytoma cell) cells (B), stained with ab4653 at 1/500 dilution (Red) and costained with chicken polyclonal anti-vimentin antibody at 1/10,000 dilution (Green). Blue: DAPI staining of nuclear DNA.

文献 (3)

发表研究结果有使用 ab4653？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4653 被引用在 3 文献中.

  • Li L  et al. Up-regulation of Cavß3 subunit in primary sensory neurons increases voltage-activated Ca2+ channel activity and nociceptive input in neuropathic pain. J Biol Chem 287:6002-13 (2012). IHC-FrFl ; Rat . PubMed: 22187436
  • Brandao KE  et al. A-kinase anchoring protein 150 expression in a specific subset of TRPV1- and CaV 1.2-positive nociceptive rat dorsal root ganglion neurons. J Comp Neurol 520:81-99 (2012). WB, ICC/IF ; Rat . PubMed: 21674494
  • Errante L  et al. The intermediate filament protein peripherin is a marker for cerebellar climbing fibres. J Neurocytol 27:69-84 (1998). PubMed: 9609398

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Peripherin antibody [8G2] - nociceptive neuron marker

 Poor
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
No
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Karine Thibault

Verified customer

提交于 Oct 17 2011

Question

Do you know anything about peripherin labeling climbing fibers in the cerebellum? Has any information been published about your peripherin antibodies being used to label climbing fibers? Does your peripherin antibody label climbing fibers?

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Answer

Thank you for your enquiry. Both antibodies do stain cerebellar climbing fibers; please see: Errante et al. The intermediate filament protein peripherin is a marker for cerebellar climbing fibres. J Neurocytol. 1998 Feb;27(2):69-84. PubMed ID 9609398. This is actually also the first paper to characterize those two antibodies. I have added this paper to the antibody datasheets for future reference. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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