AB219057

重组Anti-Periostin抗体[EPR19936]

Anti-Periostin antibody [EPR19936]

RabMAb
Recombinant
了解详情

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(4 Publications)

Rabbit Recombinant Monoclonal Periostin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.

查看别名

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

4 Images

Supplier Data

Immunoprecipitation - Anti-Periostin antibody [EPR19936] (AB219057)

Periostin was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab219057 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219057 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human fetal heart lysate 10 μg (Input).

Lane 2 : ab219057 IP in Human fetal heart lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219057 in Human fetal heart lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Periostin antibody [EPR19936] (ab219057)

Predicted band size: 93 kDa

false

Supplier Data

Western blot - Anti-Periostin antibody [EPR19936] (AB219057)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Periostin antibody [EPR19936] (AB219057)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1-3 : 30 seconds; Lane 4 : 5 seconds.

All lanes:

Western blot - Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1:

Human colon cancer lysate at 20 µg

Lane 2:

Human stomach lysate at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

Human breast cancer lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Supplier Data

Western blot - Anti-Periostin antibody [EPR19936] (AB219057)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissues : liver, pancreas (PMID : 15514205, PMID : 29163770).

This antibody is not suitable for cell line testing.

All lanes:

Western blot - Anti-Periostin antibody [EPR19936] (ab219057) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

MDA-MB-468 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 180s

不同偶联物与剂型 (1)

Carrier free

Anti-Periostin antibody [EPR19936] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19936

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody recognizes a different epitope of Periostin compared to ab219056.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "This antibody is not suitable for cell line testing.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.

Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).

See full target information POSTN

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular and clinical oncology 23:82 PubMed40692747

2025

POSTN is exclusively activated in cancer-associated fibroblasts and leads to unfavorable prognosis of patients with gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ching Hei To,Fuda Xie,Peiyao Yu,Jialin Wu,Yang Lyu,Bonan Chen,Tiejun Feng,Hoi Wing Leung,Wei Kang

International journal of biological sciences 21:3573-3596 PubMed40520021

2025

Integrative Single-Cell and Spatial Transcriptomics Analysis Reveals ECM-remodeling Cancer-associated Fibroblast-Derived POSTN as a Key Mediator in Pancreatic Ductal Adenocarcinoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Wu,Shuquan Li,Hao Yu,Sha Zhang,Liang Yan,Xiaoya Guan,Wei Xu,Zhen Wang,Ang Lv,Xiuyun Tian,Chunyi Hao,Jianhui Wu

Regenerative therapy 28:345-357 PubMed39896441

2025

Stable preparation of transplantable periodontal ligament-derived mesenchymal stem cell sheets in thermoresponsive culture dishes with tunable cell detachability.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuki Morita,Masamichi Nakayama,Jiacheng Wang,Satoru Onizuka,Masahiro Hatasa,Yujin Ohsugi,Yosuke Tsuchiya,Hiromi Niimi,Anhao Liu,Hideaki Sakai,Teruo Okano,Takanori Iwata

Kidney360 4:1562-1579 PubMed37858297

2023

Molecular Phenotyping and Mechanisms of Myocardial Fibrosis in Advanced Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gayatri Narayanan,Arvin Halim,Alvin Hu,Keith G Avin,Tzongshi Lu,Daniel Zehnder,Takashi Hato,Neal X Chen,Sharon M Moe,Kenneth Lim

View all publications

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重组Anti-Periostin抗体[EPR19936]