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AB223194

重组Anti-Periostin抗体[EPR19934] - BSA and Azide free

Anti-Periostin antibody [EPR19934] - BSA and Azide free

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Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Periostin in Western Blot, IP, IHC-P. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

6 Images
Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)
  • WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissues : liver, pancreas (PMID : 15514205, PMID : 29163770).

This antibody is not suitable for cell line testing.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (<a href='/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

MDA-MB-468 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 180s

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)
  • WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (<a href='/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID : 26023718).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID : 24273600).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)
  • IP

Supplier Data

Immunoprecipitation - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Periostin was immunoprecipitated from 0.35 mg of Human breast cancer lysate with ab219056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human breast cancer lysate 10 μg (Input).
Lane 2 : ab219056 IP in Human breast cancer lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219056 in Human breast cancer lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (<a href='/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>)

Predicted band size: 93 kDa

false

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)
  • WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] - BSA and Azide free (AB223194)

This data was developed using ab219056, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (<a href='/products/primary-antibodies/periostin-antibody-epr19934-ab219056'>ab219056</a>) at 1/1000 dilution

All lanes:

Human breast cancer lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

不同偶联物与剂型 (8)

  • Unconjugated

    Anti-Periostin antibody [EPR19934]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Periostin antibody [EPR19934]

  • 660 APC

    APC Anti-Periostin antibody [EPR19934]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Periostin antibody [EPR19934]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Periostin antibody [EPR19934]

  • 578 PE

    PE Anti-Periostin antibody [EPR19934]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Periostin antibody [EPR19934]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Periostin antibody [EPR19934]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19934

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody recognizes a different epitope of Periostin compared to ab219057.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody is not suitable for cell line testing.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

What is this antibody validated in?
Anti-Periostin antibody [EPR19934] - BSA and Azide free (ab223194) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Periostin?
Anti-Periostin [EPR19934] - BSA and Azide free (ab223194) specifically detects a band for Periostin (UniProt: Q15063) at a molecular weight of 93kDa.

Other related products
We have a range of other formats of antibody clone [EPR19934] also available for your convenience: ab219056, Carrier free - ab223194, APC - ab319228, PE - ab319376, Alexa Fluor® 488 - ab319510, Alexa Fluor® 647 - ab319623, Alexa Fluor® 594 - ab319727, Alexa Fluor® 555 - ab319869, Alexa Fluor® 750 - ab321403

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/Β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvΒ3 and αvΒ5 facilitating cell adhesion and migration. The Wnt/Β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
See full target information POSTN
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