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AB219056

重组Anti-Periostin抗体[EPR19934]

Anti-Periostin antibody [EPR19934]

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(2 Publications)

Rabbit Recombinant Monoclonal Periostin antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

查看别名

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

6 Images
Western blot - Anti-Periostin antibody [EPR19934] (AB219056)
  • WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression tissues : liver, pancreas (PMID : 15514205, PMID : 29163770).

This antibody is not suitable for cell line testing.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human pancreas tissue lysate at 20 µg

Lane 4:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

MDA-MB-468 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 180s

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)
  • WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Periostin with ab219056 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human colon (PMID : 26023718).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody [EPR19934] (AB219056)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling Periostin with ab219056 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Extracellular matrix staining on human lung cancer (PMID : 24273600).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (AB219056)
  • IP

Supplier Data

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (AB219056)

Periostin was immunoprecipitated from 0.35 mg of Human breast cancer lysate with ab219056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human breast cancer lysate 10 μg (Input).

Lane 2 : ab219056 IP in Human breast cancer lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab219056 in Human breast cancer lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Periostin antibody [EPR19934] (ab219056)

Predicted band size: 93 kDa

false

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)
  • WB

Supplier Data

Western blot - Anti-Periostin antibody [EPR19934] (AB219056)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Periostin antibody [EPR19934] (ab219056) at 1/1000 dilution

All lanes:

Human breast cancer lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 93 kDa

Observed band size: 93 kDa

false

Exposure time: 3min

不同偶联物与剂型 (8)

  • Carrier free

    Anti-Periostin antibody [EPR19934] - BSA and Azide free

  • 660 APC

    APC Anti-Periostin antibody [EPR19934]

  • 578 PE

    PE Anti-Periostin antibody [EPR19934]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Periostin antibody [EPR19934]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Periostin antibody [EPR19934]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Periostin antibody [EPR19934]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Periostin antibody [EPR19934]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Periostin antibody [EPR19934]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR19934

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody recognizes a different epitope of Periostin compared to ab219057.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p>This antibody is not suitable for cell line testing in WB</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
See full target information POSTN
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 22:1023 PubMed34373709

2021

mRNA expression level of , , , and modulates 5-fluorouracil resistance in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Liu,Rongmu Xia,Chenmeng Li,Xiaocong Chen,Xuemin Cai,Wengang Li

Cell death & disease 9:1082 PubMed30348980

2018

Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Yu,Kailiu Wu,Xu Wang,Jianjun Zhang,Lizhen Wang,Yingying Jiang,Xueqin Zhu,Wantao Chen,Ming Yan
View all publications
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详情请参阅我们的条款与条件。
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