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AB14041

Anti-Periostin 抗体

Anti-Periostin antibody

5

(20 Reviews)

|

(274 Publications)

Anti-Periostin antibody (ab14041) is a rabbit polyclonal antibody detecting Periostin in IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Chicken, Human, Mouse, Rat.

- Over 210 publications
- Trusted since 2004

查看别名

OSF2, POSTN, Periostin, PN, Osteoblast-specific factor 2, OSF-2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (AB14041)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (AB14041)

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human colon tissue section labeling Periostin with ab14041.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (AB14041)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (AB14041)

The periostin staining in prostate cancer and paired non-tumorous prostate tissues (TMA from JHU, 140 cases).

(A) The pathologists' criteria for IHC scoring.(a) 0, undetectable; (b)1+, weak staining; (c) 2+, medium staining; (d) 3+, strong staining. Periostin was mainly stained in peritumoral stroma.

Tian et al. PLoS One. 2015 Mar 17;10(3):e0121502. doi: 10.1371/journal.pone.0121502. eCollection 2015. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (AB14041)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Periostin antibody (AB14041)

ICC/IF image of ab14041 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

The cells were fixed in 100% methanol (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14041, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (AB14041)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Periostin antibody (AB14041)

Immunohistochemistry analysis of formalin-fixed paraffin-embedded human normal skin tissue section labeling Periostin with ab14041.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human, Chicken, Mouse, Rat

应用

ELISA, IHC-Fr, IHC-P, ICC/IF

applications

免疫原

Recombinant Fragment Protein within Human POSTN aa 1-700. The exact immunogen used to generate this antibody is proprietary information.

Q15063

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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产品详情

Product Specifications
Anti-Periostin antibody (ab14041) is a rabbit polyclonal antibody and is validated for use in ELISA, ICC/IF, IHC-Fr, IHC-P in chicken, human, mouse, rat samples.

Quality and Validation
Anti-Periostin antibody (ab14041) specifically detects Periostin (UniProt ID: Q15063; Molecular weight: 91kDa) and is sold in 50 µg selling sizes.
Abcam's high quality validation processes ensure Anti-Periostin antibody (ab14041) has high sensitivity and specificity.
Anti-Periostin antibody (ab14041) has been cited over 212 times in peer reviewed journals and is trusted by the scientific community.
Anti-Periostin antibody (ab14041) has 20 independent reviews from customers.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
Constituents: PBS, 0.58% Sodium chloride
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Periostin also known as POSTN or OSF-2 is an extracellular matrix protein with a molecular weight of approximately 90 kDa. It plays a mechanical role in tissue remodeling by binding to integrins which supports tissue repair and regeneration. Periostin is mainly expressed in collagen-rich tissues such as bone periodontal ligament and cardiac tissues. It is synthesized by osteoblasts fibroblasts and other stromal cells functioning as a structural protein that supports cell adhesion and migration.
Biological function summary

The periostin protein contributes to the maintenance of tissue integrity and mechanical properties. It does not solely act; it forms part of a multiprotein complex with collagen and other extracellular matrix components. This involvement is necessary for maintaining cellular interactions and promoting tissue healing processes. Additionally periostin influences cell proliferation and migration impacting developmental and wound healing biology.

Pathways

Periostin is involved in the integrin signaling pathway and the Wnt/β-catenin pathway both important for cell communication and movement. Through these pathways periostin interacts with integrins αvβ3 and αvβ5 facilitating cell adhesion and migration. The Wnt/β-catenin pathway interaction involves several proteins including Frizzled receptors ensuring regulation of cellular proliferation and differentiation. These pathways make periostin a coordinator of complex cellular processes.

The periostin protein is associated with conditions such as fibrotic diseases and cardiovascular disorders. In fibrosis periostin promotes collagen cross-linking exacerbating tissue stiffness. This involves increased interaction with proteins like collagen types I and V contributing to pathological fibrotic progression. In cardiovascular disorders elevated periostin levels after myocardial injury are linked with fibrotic scarring where it collaborates with matrix metalloproteinases to remodel the cardiac tissue architecture. Understanding these connections can lead to novel therapeutic strategies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Induces cell attachment and spreading and plays a role in cell adhesion (PubMed : 12235007). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity).
See full target information POSTN
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文献 (274)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0332531 PubMed41066485

2025

Evaluation of bone regenerative and bioactive potential of β-TCP/PLLA/PGA nanobiomaterial in conjunction with concentrated conditioned media from rapidly expanding clone (REC) of mesenchymal stem cells: A pilot animal study.

Applications

Unspecified application

Species

Unspecified reactive species

Ankhtsetseg Shijirbold,Mrunalini Ramanathan,Masako Fujioka-Kobayashi,Nithish Sankepally,Yuhei Matsuda,Yumi Matsuzaki,Yuki Fujita,Takahiro Kanno

Molecular medicine reports 32: PubMed41041835

2025

Regulatory role of POSTN in keloid pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Jiang,Fan Zhuo,Xiahong Li,Kaoyuan Zhang,Jiaxu Gu,Jingwen Wu,Weilong Zhong,Yanfen Zou,Bo Yu,Cong Huang

Scientific reports 15:25649 PubMed40665058

2025

Periostin mediates collagen production, ECM remodeling and myofibroblast differentiation in breast prosthesis capsule formation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Yang,Shumo Li,Li Bian,Xiaoming Dai,Jun Hu,Yun Ma,Zhiyuan Wang

British journal of cancer 133:633-647 PubMed40640495

2025

Cancer-associated fibroblasts are associated with neo-adjuvant treatment response in oesophageal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Robert C Walker,Stella P Breininger,Benjamin P Sharpe,Jack Harrington,Ian Reddin,Carmen Tse,Rushda Rajak,Annette Hayden,Saqib Rahman,Ben Grace,Fereshteh Izadi,Jonathan West,Maria Secrier,Zoë S Walters,Matthew J J Rose-Zerilli,Timothy J Underwood

Journal of molecular and cellular cardiology plus 12:100454 PubMed40485774

2025

Glyoxal is a superior fixative to formaldehyde in promoting antigenicity and structural integrity in murine cardiac tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Allen C T Teng,Dev Mehangrey,Ava Vandenbelt,Karl Vearncombe,Justin D Callahan,Priya Mistry,Wenping Li,Cristine J Reitz,Omar Hamed,Madison Roche,Uros Kuzmanov,Jason E Fish,Slava Epelman,Anthony O Gramolini

Journal of orthopaedic translation 52:97-115 PubMed40275883

2025

PDGF-BB improves cortical bone quality through restoring the osteogenic microenvironment in the steroid-associated osteonecrosis of rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Huijuan Cao,Keda Shi,Jing Long,Yanzhi Liu,Xiangbo Meng,Cuishan Huang,Jie Hao,Lingli Li,Yiqing Zhao,Tianluo Ye,Yuxiao Lai,Ling Qin,Xinluan Wang

Nature communications 16:2895 PubMed40148319

2025

CD248-targeted BBIR-T cell therapy against late-activated fibroblasts in cardiac repair after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Haiting Chen,Ke Hu,Qi Tang,Junzhuo Wang,Qianyu Gu,Jiayu Chen,Jiaxin Hu,Ningxin Peng,Meng Guo,Yaohui Jiang,Qingbo Xu,Jun Xie

EMBO reports 26:1472-1503 PubMed39953252

2025

LATS1/2 inactivation in the mammary epithelium drives the evolution of a tumor-associated niche.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph G Kern,Lina Kroehling,Anthony J Spinella,Stefano Monti,Xaralabos Varelas

Communications biology 8:216 PubMed39934335

2025

Long QT syndrome type 3 gain-of-function of Na1.5 increases ventricular fibroblasts proliferation and pro-fibrotic factors.

Applications

Unspecified application

Species

Unspecified reactive species

Claire Castro,Justine Patin,Cyrielle Jajkiewicz,Franck Chizelle,Cynthia Ore Cerpa,Agnès Tessier,Eva Le Pogam,Imen Fellah,Isabelle Baró,Flavien Charpentier,Mickaël Derangeon

JCI insight 10: PubMed39688917

2024

Negative feedback between PTH1R and IGF1 through the Hedgehog pathway in mediating craniofacial bone remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Fan,Ping Lyu,Jiahe Wang,Yali Wei,Zucen Li,Shiwen Zhang,Takehito Ouchi,Junjun Jing,Quan Yuan,Clifford J Rosen,Chenchen Zhou
View all publications
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