Anti-Perilipin 2抗体(ab78920)
Key features and details
- Rabbit polyclonal to Perilipin-2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Perilipin 2抗体
参阅全部 Perilipin-2 一抗 -
描述
兔多克隆抗体to Perilipin-2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Pig, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Ab78920 gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293. ICC/IF: HepG2 cell line, Raw264.7 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab78920于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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| 说明 |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
靶标
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功能
May be involved in development and maintenance of adipose tissue. -
组织特异性
Milk lipid globules. -
序列相似性
Belongs to the perilipin family. -
翻译后修饰
Acylated; primarily with C14, C16 and C18 fatty acids. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 123 Human
- Entrez Gene: 11520 Mouse
- Omim: 103195 Human
- SwissProt: Q99541 Human
- SwissProt: P43883 Mouse
- SwissProt: Q4PLW0 Pig
- Unigene: 3416 Human
- Unigene: 381 Mouse
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别名
- ADFP antibody
- Adipophilin antibody
- Adipose differentiation related protein antibody
see all
图片
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Western blot - Anti-Perilipin 2 antibody (ab78920)All lanes : Anti-Perilipin 2 antibody (ab78920) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Additional bands at: 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-Perilipin 2 antibody (ab78920)ab78920 staining Perilipin-2 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab78920 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-Perilipin 2 antibody (ab78920)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab78920 被引用在 15 文献中.
- Li MH et al. Characterization of a novel Hoxa5eGFP mouse line. Dev Dyn 252:536-546 (2023). PubMed: 36577717
- Leznicki P et al. Co-translational biogenesis of lipid droplet integral membrane proteins. J Cell Sci 135:N/A (2022). PubMed: 34558621
- Ippolito L et al. Lactate Rewires Lipid Metabolism and Sustains a Metabolic-Epigenetic Axis in Prostate Cancer. Cancer Res 82:1267-1282 (2022). PubMed: 35135811
- Pillai S et al. Lipogenesis mediated by OGR1 regulates metabolic adaptation to acid stress in cancer cells via autophagy. Cell Rep 39:110796 (2022). PubMed: 35545051
- Brandenburg J et al. WNT6/ACC2-induced storage of triacylglycerols in macrophages is exploited by Mycobacterium tuberculosis. J Clin Invest 131:N/A (2021). PubMed: 34255743