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Cardiovascular Lipids / Lipoproteins Adipose Related Lipid Droplet Protein

Anti-Perilipin-1抗体(ab3526)

  • Datasheet
  • SDS
Reviews (16)Q&A (3)References (86)

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Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
  • Western blot - Anti-Perilipin-1 antibody (ab3526)

Key features and details

  • Rabbit polyclonal to Perilipin-1
  • Suitable for: Flow Cyt, IHC-P, WB, ICC, IHC-FoFr
  • Reacts with: Mouse, Rat, Cow, Dog, Human, Rhesus monkey
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Perilipin-1 antibody [EPR3753(2)] (ab172907)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Perilipin-1抗体
    参阅全部 Perilipin-1 一抗
  • 描述

    兔多克隆抗体to Perilipin-1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: Flow Cyt, IHC-P, WB, ICC, IHC-FoFrmore details
  • 种属反应性

    与反应: Mouse, Rat, Cow, Dog, Human, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
    (Peptide available as ab5009)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • ICC: mouse 3T3-L1 cells; WB: mouse 3T3-L1 cells
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Lipids / Lipoproteins
    • Adipose Related
    • Lipid Droplet Protein
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cardiovascular
    • Atherosclerosis
    • Lipoprotein metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipoprotein metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Types of disease
    • Heart disease
    • Neuroscience
    • Development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Immunizing Peptide (Blocking)

    • Perilipin-1 peptide (ab5009)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3526于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use at an assay dependent concentration.
IHC-P (6)
Use at an assay dependent concentration.
WB (2)
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).

By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract.

ICC
Use a concentration of 2 µg/ml.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20049087
说明
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).

By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract.

ICC
Use a concentration of 2 µg/ml.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20049087

靶标

  • 功能

    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • 组织特异性

    Adipocytes.
  • 序列相似性

    Belongs to the perilipin family.
  • 翻译后修饰

    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • 细胞定位

    Lipid droplet. Lipid droplet surface-associated.
  • Target information above from: UniProt accession O60240 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 520598 Cow
    • Entrez Gene: 5346 Human
    • Entrez Gene: 103968 Mouse
    • Entrez Gene: 25629 Rat
    • Omim: 170290 Human
    • SwissProt: O60240 Human
    • SwissProt: Q8CGN5 Mouse
    • SwissProt: P43884 Rat
    • Unigene: 103253 Human
    • Unigene: 254917 Mouse
    • Unigene: 9737 Rat
    see all
  • 别名

    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

图片

  • Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
    Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)

    Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).

    Perilipin A was labeled with ab3526 at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel A: green).

    Panel B: Nuclei were stained with DAPI (blue); Panel C: (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1:300. Panel D: merged image showing cytosolic localization; Panel E: untreated; Panel F: control cells with no primary antibody.

  • Western blot - Anti-Perilipin-1 antibody (ab3526)
    Western blot - Anti-Perilipin-1 antibody (ab3526)

    Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (86)

发表研究结果有使用 ab3526?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3526 被引用在 86 文献中.

  • Hariri H  et al. Ubiquitin specific peptidase Usp53 regulates osteoblast versus adipocyte lineage commitment. Sci Rep 11:8418 (2021). PubMed: 33875709
  • Gantov M  et al. Beige adipocytes contribute to breast cancer progression. Oncol Rep 45:317-328 (2021). PubMed: 33416183
  • Mori H  et al. The molecular and metabolic program by which white adipocytes adapt to cool physiologic temperatures. PLoS Biol 19:e3000988 (2021). PubMed: 33979328
  • Liu B  et al. Inhibition of Notch Signaling Promotes the Differentiation of Epicardial Progenitor Cells into Adipocytes. Stem Cells Int 2021:8859071 (2021). PubMed: 33897781
  • Pievani A  et al. Acute myeloid leukemia shapes the bone marrow stromal niche in vivo. Haematologica 106:865-870 (2021). PubMed: 32381570
View all Publications for this product

客户评价及客户问答

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1-10 of 19 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Perilipin-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Apr 06 2022

Western blot abreview for Anti-Perilipin-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Dec 07 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Perilipin-1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice liver tissue section)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice liver tissue section
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Aug 06 2021

IHC - Wholemount abreview for Anti-Perilipin-1 antibody

Good
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Tissue (Hepatocyte spheroid)
Specification
Hepatocyte spheroid
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 19 2021

Western blot abreview for Anti-Perilipin-1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Primary hepatocytes)
Gel Running Conditions
Reduced Denaturing (12 %)
Loading amount
30 µg
Treatment
with 500uM free fatty acids
Specification
Primary hepatocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jul 19 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Perilipin-1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Fhs74int)
Specification
Fhs74int
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 10 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Perilipin-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (vertebrae bodies)
Permeabilization
Yes - trixton X-100
Specification
vertebrae bodies
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
10%NBF
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 21 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Perilipin-1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Triton
Specification
Bone
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Tina Yuan

Verified customer

提交于 Sep 30 2019

Immunohistochemistry (Resin sections) abreview for Anti-Perilipin-1 antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Resin sections)
Sample
Human Tissue sections (adipocytes)
Specification
adipocytes
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. M Malatesta

Verified customer

提交于 Dec 01 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-Perilipin-1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (fat tissue)
Permeabilization
No
Specification
fat tissue
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 14 2017

1-10 of 19 Abreviews or Q&A

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