Anti-Perilipin-1抗体(ab3526)
Key features and details
- Rabbit polyclonal to Perilipin-1
- Suitable for: Flow Cyt, IHC-P, WB, ICC, IHC-FoFr
- Reacts with: Mouse, Rat, Cow, Dog, Human, Rhesus monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Perilipin-1抗体
参阅全部 Perilipin-1 一抗 -
描述
兔多克隆抗体to Perilipin-1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt, IHC-P, WB, ICC, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat, Cow, Dog, Human, Rhesus monkey -
免疫原
Synthetic peptide corresponding to Rat Perilipin-1 aa 500-600.
(Peptide available asab5009) -
阳性对照
- ICC: mouse 3T3-L1 cells; WB: mouse 3T3-L1 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
|
| IHC-P | (6) |
Use at an assay dependent concentration.
|
| WB | (2) |
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009).
By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
| ICC |
Use a concentration of 2 µg/ml.
|
|
| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 20049087
|
| 说明 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/4000. Can be blocked with Perilipin-1 peptide (ab5009). By Western blot, this antibody detects an ~62 kDa protein representing Perilipin-1 from 3T3-L1 cell extract. |
|
ICC
Use a concentration of 2 µg/ml. |
|
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20049087 |
靶标
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功能
Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. -
组织特异性
Adipocytes. -
序列相似性
Belongs to the perilipin family. -
翻译后修饰
Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet. -
细胞定位
Lipid droplet. Lipid droplet surface-associated. - Information by UniProt
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数据库链接
- Entrez Gene: 520598 Cow
- Entrez Gene: 5346 Human
- Entrez Gene: 103968 Mouse
- Entrez Gene: 25629 Rat
- Omim: 170290 Human
- SwissProt: O60240 Human
- SwissProt: Q8CGN5 Mouse
- SwissProt: P43884 Rat
see all -
别名
- Lipid droplet associated protein antibody
- Lipid droplet-associated protein antibody
- PERI antibody
see all
图片
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Immunocytochemistry - Anti-Perilipin-1 antibody (ab3526)
Immunocytochemistry analysis of 3T3-L1 cells (Mouse embryonic fibroblast cell line), 4% paraformaldehyde fixed (10 minutes) permeabilized with 0.1% Triton™ X-100 (10 minutes), and blocked with 1% BSA for (1 hour).
Perilipin A was labeled with ab3526 at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature with secondary antibody: Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel A: green).
Panel B: Nuclei were stained with DAPI (blue); Panel C: (red) F-actin stained with Alexa Fluor® 555 Rhodamine Phalloidin 1:300. Panel D: merged image showing cytosolic localization; Panel E: untreated; Panel F: control cells with no primary antibody.
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Western blot - Anti-Perilipin-1 antibody (ab3526)Western blot detection of Perilipin-1 in 3T3-L1 cell extract using ab3526.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (111)
ab3526 被引用在 111 文献中.
- Karadeniz F et al. Dracunculin Inhibits Adipogenesis in Human Bone Marrow-Derived Mesenchymal Stromal Cells by Activating AMPK and Wnt/β-Catenin Signaling. Int J Mol Sci 23:N/A (2022). PubMed: 35054838
- Zhao Y et al. Expression of Recombinant Rat Secretable FNDC5 in Pichia Pastoris and Detection of Its Biological Activity. Front Endocrinol (Lausanne) 13:852015 (2022). PubMed: 35321332
- Gautheron J et al. Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes. BMC Med 20:95 (2022). PubMed: 35341481
- Niu X et al. Preoperative Short-term High-Carbohydrate Diet Provides More High-Quality Transplantable Fat and Improves the Outcome of Fat Grafts in Mice. Aesthet Surg J 42:NP531-NP545 (2022). PubMed: 35460566
- Tang W et al. Investigating the Adipogenic Effects of Different Tissue-Derived Decellularized Matrices. Front Bioeng Biotechnol 10:872897 (2022). PubMed: 35497363