重组Anti-Pericentrin抗体[mAbcam 28144] - Centrosome Marker (ab28144)
![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.cn/ps/products/28/ab28144/Images/ab28144-413145-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunocytochemistry-hela-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
- Suitable for: ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Pericentrin抗体[mAbcam 28144] - Centrosome Marker
参阅全部 Pericentrin 一抗 -
描述
小鼠单克隆抗体[mAbcam 28144] to Pericentrin - Centrosome Marker -
宿主
Mouse -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Chinese hamster
不与反应:
Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HeLa cells.
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常规说明
This product has switched from a hybridoma to recombinant production method on 9th September 2020.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
mAbcam 28144 -
骨髓瘤
Sp2/0 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28144于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (11) |
1/50.
|
| 说明 |
|---|
|
ICC/IF
1/50. |
靶标
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功能
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
组织特异性
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
疾病相关
Microcephalic osteodysplastic primordial dwarfism 2 -
结构域
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
细胞定位
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 5116 Human
- Omim: 605925 Human
- SwissProt: O95613 Human
- Unigene: 474069 Human
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别名
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
Immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab28144 at 1/50 dilution at 4°C overnight, followed by AlexaFluor® 488-conjugated Goat anti-Rabbit secondary
antibody (ab150077) at 1/1000 dilution at RT for 30min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] (ab195889) was used as a counterstain at 1/200 dilution at RT for 30min. Nucleus were visualized using DAPI. The cells were 4% PFA fixed and permeabilized with 0.1% Triton X-100.Confocal image showing centrosome staining in HeLa cells.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.cn/ps/products/28/ab28144/Images/ab28144-275774-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)Image from Sikkema, William K. A. et al. PLoS ONE 9.6 (2014): e98838. PMC. Web. 24 Feb. 2017. doi: 10.1371/journal.pone.0098838. Fig 4F. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).
This image was generated from the hybridoma version.
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![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.cn/ps/products/28/ab28144/Images/ab28144-275773-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)This image is courtesy of an anonymous abreview.Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.
This image was generated from the hybridoma version. -
![Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.cn/ps/products/28/ab28144/Images/ab28144-275770-anti-pericentrin-antibody-mabcam-28144-centrosome-marker-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)This image is courtesy of an Abreview submitted by Dr Kirk McManus, Univ. of Manitoba/Cancer Care MICB.Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.
This image was generated from the hybridoma version.
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![Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)](https://www.abcam.cn/ps/products/28/ab28144/Images/ab28144-13-benefits-of-recombinant-antibodies.png)
Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (96)
ab28144 被引用在 96 文献中.
- Meyer N et al. Fetal endothelial colony-forming cell impairment after maternal kidney transplantation. Pediatr Res 93:810-817 (2023). PubMed: 35732823
- Saint-Dizier F et al. Discovery of 2-(3-Benzamidopropanamido)thiazole-5-carboxylate Inhibitors of the Kinesin HSET (KIFC1) and the Development of Cellular Target Engagement Probes. J Med Chem 66:2622-2645 (2023). PubMed: 36749938
- Gregorczyk M et al. Functional characterization of C21ORF2 association with the NEK1 kinase mutated in human in diseases. Life Sci Alliance 6:N/A (2023). PubMed: 37188479
- Cunningham A et al. Combination Effects of Integrin-linked Kinase and Abelson Kinase Inhibition on Aberrant Mitosis and Cell Death in Glioblastoma Cells. Biology (Basel) 12:N/A (2023). PubMed: 37508338
- Yoshizawa K et al. Tetraploidy-linked sensitization to CENP-E inhibition in human cells. Mol Oncol 17:1148-1166 (2023). PubMed: 36688680