AB220784

重组Anti-Pericentrin抗体[EPR21987] - Centrosome Marker

Name: Pericentrin抗体[EPR21987] - Centrosome Marker (ab220784)| Abcam中文官网
Brand: Abcam Limited
SKU: AB220784
Price: 2918 CNY
Availability: InStock
Rating: 4 (2 reviews)

Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

RabMAb
Recombinant
Lab Essentials
了解详情

(2 Reviews)

(16 Publications)

Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784) is a rabbit monoclonal antibody detecting Pericentrin in Flow Cytometry (Intra), IP, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

查看别名

KIAA0402, PCNT2, PCNT, Pericentrin, Kendrin, Pericentrin-B

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human mammary epithelial cells (PMID : 16534625, PMID : 22031837). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human thyroid carcinoma (PMID : 16534625, PMID : 22031837). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pericentrin with ab220784 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Immunofluorescent analysis of 100% Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human pancreas (PMID : 16534625, PMID : 22031837). Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (AB220784)

Immunofluorescent analysis of 100% Methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

不同偶联物与剂型 (6)

Carrier free

Anti-Pericentrin antibody [EPR21987] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Pericentrin - Centrosome Marker antibody [EPR21987]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker
578 PE

PE Anti-Pericentrin antibody [EPR21987] - Centrosome Marker

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21987

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Other related products
We have a range of other formats of antibody clone [EPR21987] also available for your convenience: ab220784, Carrier free - ab237034, Alexa Fluor® 488 - ab270118, Alexa Fluor® 647 - ab270119, Alexa Fluor® 594 - ab314881, Alexa Fluor® 555 - ab319181, PE - ab322452

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pericentrin also known as PCNT is a large protein with a mass of approximately 360 kDa. It primarily localizes at the centrosome an organelle serving as the main microtubule organizing center in cells. Expressed in most cell types pericentrin is an essential structural component of the centrosome. Researchers often use pericentrin staining techniques to visualize centrosomes aiding in the study of cell division. This protein functions prominently as a centrosome marker facilitating centrosome image and imaging of centrosome-related structures.

Biological function summary

Pericentrin contributes significantly to maintaining centrosome integrity and organizing microtubules during cell division. It is a part of the pericentriolar material a complex that stabilizes and anchors microtubules to the centrosome. By forming a scaffold at the centrosome pericentrin plays an important role in recruiting other proteins like γ-tubulin enhancing its function in nucleating microtubules. The pericentrin protein is vital for proper spindle organization during mitosis affecting accurate chromosome segregation.

Pathways

Pericentrin is integral to the cell cycle and mitotic spindle assembly pathways. It interacts with several other proteins involved in mitosis including γ-tubulin and ninein to manage the assembly and stability of the mitotic spindle. By participating in these pathways pericentrin ensures correct cellular replication and division therefore maintaining genetic stability within organisms.

Mutations in the pericentrin gene have been linked to microcephalic osteodysplastic primordial dwarfism type II and Seckel syndrome. These conditions suggest a connection between defective pericentrin function and disruptions in cell division and growth. Studies also show that alterations in pericentrin levels can influence tumorigenesis by affecting centrosome function and chromosome segregation increasing the importance of this protein in cancer research.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.

See full target information PCNT

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

Inflammation : PubMed40764471

2025

WFS1 Gene Mutation (c.2389G > A) Induces Immune Disorders by Promoting DC Maturation through Inhibition of TMEM176A.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Lin,Yuanhui Qiu,Chenyinuo Tan,Ziyu Liu,Yi Yang,Yijie He,Jiarui Zhao,Jing Cheng,Zhaopeng Wang,Minghao Li,Lijie Wang,Mengqi Wang,Min Zhang

Science advances 11:eadu5668 PubMed40749049

2025

eIF2A regulates cell migration in a translation-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Jungfleisch,Neus Mestre-Farràs,Raúl Gómez-Riera,Oriane Pourcelot,Edouard Bertrand,Nadia Halidi,Fátima Gebauer

The EMBO journal 44:2976-2996 PubMed40229407

2025

Glutamylation of centrosomes ensures their function by recruiting microtubule nucleation factors.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Rong Hong,Yi-Chien Chuang,Wen-Ting Yang,Chiou-Shian Song,Hung-Wei Yeh,Bing-Huan Wu,I-Hsuan Lin,Po-Chun Chou,Shiau-Chi Chen,Lohitaksh Sharma,Jui-Chen Lu,Rou-Ying Li,Ya-Chu Chang,Kuan-Ju Liao,Hui-Chun Cheng,Won-Jing Wang,Lily Hui-Ching Wang,Yu-Chun Lin

Cells 14: PubMed39996710

2025

NE-MTOC Formation in Skeletal Muscle Is Mbnl2-Dependent and Occurs in a Sequential and Gradual Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Payel Das,Robert Becker,Silvia Vergarajauregui,Felix B Engel

Nature communications 15:8273 PubMed39333556

2024

Lipid droplets sequester palmitic acid to disrupt endothelial ciliation and exacerbate atherosclerosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjie Tan,Zhenzhou Huang,Yi Jin,Jiaying Wang,Hongjun Fan,Yangyang Liu,Liang Zhang,Yue Wu,Peiwei Liu,Tianliang Li,Jie Ran,He Tian,Sin Man Lam,Min Liu,Jun Zhou,Yunfan Yang

Life science alliance 7: PubMed39074902

2024

Kinesins regulate the heterogeneity in centrosome clustering after whole-genome duplication.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Ty Lau,Hoi Tang Ma,Randy Yc Poon

Scientific reports 14:13727 PubMed38877142

2024

Loss of connectin novex-3 leads to heart dysfunction associated with impaired cardiomyocyte proliferation and abnormal nuclear mechanics.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Hashimoto,Momoko Ohira,Aya Kodama,Misaki Kimoto,Mariko Inoue,Shigenobu Toné,Yuu Usui,Akira Hanashima,Takato Goto,Yuhei Ogura,Yoshihiro Ujihara,Satoshi Mohri

Molecular cancer research : MCR 22:423-439 PubMed38324016

2024

Coregulation of NDC80 Complex Subunits Determines the Fidelity of the Spindle-Assembly Checkpoint and Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sehong Kim,Thomas T Y Lau,Man Kit Liao,Hoi Tang Ma,Randy Y C Poon

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23307 PubMed37983646

2023

Iron inhibits glioblastoma cell migration and polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesh Shenoy,Sina Kheirabadi,Zaman Ataie,Aurosman Pappus Sahu,Kondaiah Palsa,Quinn Wade,Chachrit Khunsriraksakul,Vladimir Khristov,Becky Slagle-Webb,Justin D Lathia,Hong-Gang Wang,Amir Sheikhi,James R Connor

EMBO reports 24:e56870 PubMed37971148

2023

Circadian oscillation in primary cilium length by clock genes regulates fibroblast cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Nakazato,Yuki Matsuda,Faryal Ijaz,Koji Ikegami

View all publications

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重组Anti-Pericentrin抗体[EPR21987] - Centrosome Marker