重组Anti-Pericentrin抗体[EPR21987] - Centrosome Marker (ab220784)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21987] to Pericentrin - Centrosome Marker
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), IP
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Pericentrin抗体[EPR21987] - Centrosome Marker
参阅全部 Pericentrin 一抗 -
描述
兔单克隆抗体[EPR21987] to Pericentrin - Centrosome Marker -
宿主
Rabbit -
特异性
IHC is recommended for human only. -
经测试应用
适用于: IHC-P, ICC/IF, Flow Cyt (Intra), IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HeLa and NIH/3T3 cells. IHC-P: Human breast, pancreas and thyroid carcinoma tissues. Flow Cyt (intra): HeLa cells. IP: HepG2 and NIH/3T3 whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21987 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
- Alexa Fluor® 488 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab270118)
- Alexa Fluor® 647 Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab270119)
- Alexa Fluor® 594 Anti-Pericentrin - Centrosome Marker antibody [EPR21987] (ab314881)
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Compatible Secondaries
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab220784于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF | (1) |
1/2000.
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Flow Cyt (Intra) |
1/60.
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IP |
1/30.
|
说明 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/2000. |
Flow Cyt (Intra)
1/60. |
IP
1/30. |
靶标
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功能
Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. -
组织特异性
Expressed in all tissues tested, including placenta, liver, kidney and thymus. -
疾病相关
Microcephalic osteodysplastic primordial dwarfism 2 -
结构域
Composed of a coiled-coil central region flanked by non-helical N- and C-terminals. -
细胞定位
Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 5116 Human
- Entrez Gene: 18541 Mouse
- Omim: 605925 Human
- SwissProt: O95613 Human
- SwissProt: P48725 Mouse
- Unigene: 474069 Human
- Unigene: 251794 Mouse
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别名
- Centrosome Marker antibody
- Ken antibody
- Kendrin antibody
see all
图片
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Immunofluorescent analysis of 100% Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Immunofluorescent analysis of 100% Methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Pericentrin was immunoprecipitated from 0.35 mg HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 10 μg (input).
Lane 2: ab220784 IP in HepG2 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in HepG2 whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 30 secondsThe 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We used a high sensitivity ECL substrate to develop this blot.
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Pericentrin was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection 1/1000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 μg (input).
Lane 2: ab220784 IP in NIH/3T3 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in NIH/3T3 whole cell lysate (-).Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 30 secondsThe 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We use high sensitive ECL substrate.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pericentrin with ab220784 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human mammary epithelial cells (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human pancreas (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human thyroid carcinoma (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab220784 被引用在 5 文献中.
- Schneider MWG et al. A mitotic chromatin phase transition prevents perforation by microtubules. Nature 609:183-190 (2022). PubMed: 35922507
- Rizaldy D et al. Increase in primary cilia in the epidermis of patients with atopic dermatitis and psoriasis. Exp Dermatol 30:792-803 (2021). PubMed: 33455013
- Murase Y et al. Inhibitor library screening identifies ispinesib as a new potential chemotherapeutic agent for pancreatic cancers. Cancer Sci 112:4641-4654 (2021). PubMed: 34510663
- Lau HW et al. Quantitative differences between cyclin-dependent kinases underlie the unique functions of CDK1 in human cells. Cell Rep 37:109808 (2021). PubMed: 34644583
- Vergarajauregui S et al. AKAP6 orchestrates the nuclear envelope microtubule-organizing center by linking golgi and nucleus via AKAP9. Elife 9:N/A (2020). PubMed: 33295871