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Cell Biology Cell Cycle Cell Division Spindle

Anti-Pericentrin抗体- Centrosome Marker (ab4448)

  • Datasheet
  • SDS
Reviews (34)Q&A (5)References (428)

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Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

Key features and details

  • Rabbit polyclonal to Pericentrin - Centrosome Marker
  • Suitable for: ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

选择批间可重复性更高的重组抗体

Product image
Anti-Pericentrin antibody [EPR21987] - Centrosome Marker (ab220784)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Pericentrin抗体- Centrosome Marker
    参阅全部 Pericentrin 一抗
  • 描述

    兔多克隆抗体to Pericentrin - Centrosome Marker
  • 宿主

    Rabbit
  • 特异性

    This antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2).
  • 经测试应用

    适用于: ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, African green monkey
  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: Human coronary artery endothelial, MCF7, HeLa and NIH/3T3 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Centrosome

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4448于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (29)
Use a concentration of 0.1 - 0.5 µg/ml.
说明
ICC/IF
Use a concentration of 0.1 - 0.5 µg/ml.

靶标

  • 功能

    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • 组织特异性

    Expressed in all tissues tested, including placenta, liver, kidney and thymus.
  • 疾病相关

    Microcephalic osteodysplastic primordial dwarfism 2
  • 结构域

    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • 细胞定位

    Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Target information above from: UniProt accession O95613 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5116 Human
    • Entrez Gene: 18541 Mouse
    • Omim: 605925 Human
    • SwissProt: O95613 Human
    • SwissProt: P48725 Mouse
    • Unigene: 474069 Human
    • Unigene: 251794 Mouse
    • 别名

      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

      ab4448 staining Pericentrin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)

      ab4448 staining Pericentrin in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab4448 at 0.1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 100% methanol (5 min).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)Ruppenthal et al PLoS One. 2018 Jan 25;13(1):e0191734. doi: 10.1371/journal.pone.0191734. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      A representative panel of indirect immunofluorescence microscopic images shows normal (regular, n ≤ 2) and aberrant centrosome numbers (n > 2) in interphase cells.

      Centrosomes were stained using anti-pericentrin antibody ab4448 (magenta), nuclear DNA is shown in blue (DAPI).

      Statistical methods: Kruskal-Wallis test. Mann-Whitney U tests followed by Bonferroni-Holm p-value correction were made as post-hoc tests in order to compare the MDS and sAML patients with the control group.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Gordon Chan, University of Alberta

      IF staining of pericentrin in MCF7 (Human breast adenocarcinoma cell line) cells.

      The top panel is an interphase cell showing centrosome staining. 

      The bottom panel shows a mitotic cell with spindle pole staining. 

      ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).

      Top panel - 630X magnification; Bottom panel -1000X magnification.

      The secondary antibody was Alexa-Fluor®488 anti-rabbit.

    • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)
      Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody - Centrosome Marker (ab4448)This image is courtesy of Roberto Giambruno, Marilena Ciciarello and Patrizia Lavia

      NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature.

      The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.

       

       

    实验方案

    • Recommended ICC/IF protocol with ab4448

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (428)

    发表研究结果有使用 ab4448?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4448 被引用在 428 文献中.

    • Lo YH  et al. Galectin-8 Is Upregulated in Keratinocytes by IL-17A and Promotes Proliferation by Regulating Mitosis in Psoriasis. J Invest Dermatol 141:503-511.e9 (2021). PubMed: 32805218
    • Chen TY  et al. Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response. Cell Death Differ 28:1865-1879 (2021). PubMed: 33462409
    • Chen A  et al. CRISPR/Cas9 screening identifies a kinetochore-microtubule dependent mechanism for Aurora-A inhibitor resistance in breast cancer. Cancer Commun (Lond) 41:121-139 (2021). PubMed: 33471959
    • Balestra FR  et al. TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin. Elife 10:N/A (2021). PubMed: 33491649
    • Iruzubieta P  et al. Primary cilia presence and implications in bladder cancer progression and invasiveness. Histochem Cell Biol 155:547-560 (2021). PubMed: 33495938
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 39 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

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    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human retinal pigment epithelial-1 (RPE1))
    Specification
    Human retinal pigment epithelial-1 (RPE1)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Feb 27 2023

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Endothelial cells)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    Human Endothelial cells
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Jun 23 2022

    Western blot abreview for Anti-Pericentrin antibody - Centrosome Marker

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons, HEK293T and HeLa)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons, HEK293T and HeLa
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Nov 20 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

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    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human coronary artery endothelial cells HCAEC)
    Permeabilization
    Yes - 0.1% Trition X-100 in HBSS
    Specification
    Human coronary artery endothelial cells HCAEC
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Feb 19 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

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    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HAP1)
    Permeabilization
    Yes - 0.5% Triton 5 minutes
    Specification
    HAP1
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Dec 14 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (colon carcinoma cells)
    Permeabilization
    Yes - Triton
    Specification
    colon carcinoma cells
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Sep 11 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

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    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (BS-C-1 cells)
    Permeabilization
    Yes - 0.2% Triton X
    Specification
    BS-C-1 cells
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Apr 09 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (brain section)
    Specification
    brain section
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Dec 18 2017

    Western blot abreview for Anti-Pericentrin antibody - Centrosome Marker

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing (4-12 Bis tris)
    Loading amount
    5e+006 cells
    Specification
    293T
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Mar 18 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Pericentrin antibody - Centrosome Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080)
    Permeabilization
    Yes - 0.5% Triton X100
    Specification
    HT1080
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    提交于 Mar 18 2016

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