Anti-Pericentrin 1/FROUNT抗体(ab176801)
Key features and details
- Rabbit polyclonal to Pericentrin 1/FROUNT
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Pericentrin 1/FROUNT抗体
参阅全部 Pericentrin 1/FROUNT 一抗 -
描述
兔多克隆抗体to Pericentrin 1/FROUNT -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human Pericentrin 1/FROUNT aa 1-50. The exact sequence is proprietary. (NP_079120.1).
Sequence:MEELDGEPTVTLIPGVNSKKNQMYFDWGPGEMLVCETSFNKKEKSEMVPS
Database link: Q9BW27 -
阳性对照
- 293T, HeLa, Jurkat, TCMK-1 and NIH 3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176801 was affinity purified using an epitope specific to Pericentrin 1/FROUNT immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176801于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 75 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 75 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. -
序列相似性
Belongs to the nucleoporin Nup85 family. -
细胞定位
Nucleus > nuclear pore complex. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Nucleus membrane. During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells. - Information by UniProt
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数据库链接
- Entrez Gene: 454876 Chimpanzee
- Entrez Gene: 101129029 Gorilla
- Entrez Gene: 79902 Human
- Entrez Gene: 445007 Mouse
- Entrez Gene: 100443348 Orangutan
- Entrez Gene: 287830 Rat
- Entrez Gene: 705628 Rhesus monkey
- Omim: 170285 Human
see all -
别名
- 85 kDa nucleoporin antibody
- FLJ12549 antibody
- FROUNT antibody
see all
图片
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Western blot - Anti-Pericentrin 1/FROUNT antibody (ab176801)All lanes : Anti-Pericentrin 1/FROUNT antibody (ab176801) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 75 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-Pericentrin 1/FROUNT antibody (ab176801)Detection of Pericentrin 1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab176801 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds..
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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