Anti-Perfringolysin O抗体(ab225685)
Key features and details
- Rabbit polyclonal to Perfringolysin O
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Perfringolysin O抗体 -
描述
兔多克隆抗体to Perfringolysin O -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment
预测可用于: Clostridium perfringens
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免疫原
Recombinant full length protein corresponding to Clostridium perfringens Perfringolysin O aa 1 to the C-terminus.
Database link: P0C2E9 -
阳性对照
- WB: Recombinant Clostridium perfringens Perfringolysin O protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab225685于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| 说明 |
|---|
|
WB
1/500 - 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
靶标
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相关性
Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A conformation change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol may be required for binding to host cell membranes, membrane insertion and pore formation. Can be reversibly inactivated by oxidation. -
别名
- pfo antibody
- Theta toxin antibody
- Thiol activated cytolysin antibody
图片
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Western blot - Anti-Perfringolysin O antibody (ab225685)All lanes : Anti-Perfringolysin O antibody (ab225685) at 1/500 dilution
Lane 1 : Recombinant Clostridium perfringens Perfringolysin O protein at 0.08 µg
Lane 2 : Recombinant Clostridium perfringens Perfringolysin O protein at 0.04 µg
Lane 3 : Recombinant Clostridium perfringens Perfringolysin O protein at 0.02 µg
Lane 4 : Recombinant Clostridium perfringens Perfringolysin O protein at 0.01 µg
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab225685 尚未被引用在任何文献中。