Anti-Peregrin/BRPF1抗体- N-terminal (ab226909)
Key features and details
- Rabbit polyclonal to Peregrin/BRPF1 - N-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Peregrin/BRPF1抗体- N-terminal
参阅全部 Peregrin/BRPF1 一抗 -
描述
兔多克隆抗体to Peregrin/BRPF1 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow
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免疫原
Recombinant fragment within Human Peregrin/BRPF1 (N terminal). The exact sequence is proprietary.
Database link: P55201 -
阳性对照
- WB: Peregrin/BRPF1 transfected HEK-293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226909于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 137 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 137 kDa. |
靶标
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功能
Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. Positively regulates the transcription of RUNX1 and RUNX2. -
组织特异性
High levels in testis. -
序列相似性
Contains 1 bromo domain.
Contains 1 C2H2-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain. -
翻译后修饰
Acetylated by KAT6A. -
细胞定位
Nucleus. Cytoplasm. Localization to the nucleus depends on KAT6A, ING5 and MEAF6. - Information by UniProt
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数据库链接
- Entrez Gene: 427590 Chicken
- Entrez Gene: 535775 Cow
- Entrez Gene: 7862 Human
- Entrez Gene: 78783 Mouse
- Entrez Gene: 309647 Rat
- Omim: 602410 Human
- SwissProt: P55201 Human
- Unigene: 1004 Human
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别名
- BR140 antibody
- Bromodomain and PHD finger containing 1 antibody
- Bromodomain and PHD finger-containing protein 1 antibody
see all
图片
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Western blot - Anti-Peregrin/BRPF1 antibody - N-terminal (ab226909)All lanes : Anti-Peregrin/BRPF1 antibody - N-terminal (ab226909) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : BRPF1 knockout HEK-293T cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 137 kDaFalse colour image of Western blot: Anti-Peregrin/BRPF1 antibody - N-terminal staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab226909 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 160, 145 and 138 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line ab266549 (knockout cell lysate ab258795). To generate this image, wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Observed bands - 160, 145 and 138 kDa
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Western blot - Anti-Peregrin/BRPF1 antibody - N-terminal (ab226909)All lanes : Anti-Peregrin/BRPF1 antibody - N-terminal (ab226909) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : Peregrin/BRPF1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 137 kDa -
Western blot - Anti-Peregrin/BRPF1 antibody - N-terminal (ab226909)All lanes : Anti-Peregrin/BRPF1 antibody - N-terminal (ab226909) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : Peregrin/BRPF1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Predicted band size: 137 kDa7.5% SDS PAGE gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226909 尚未被引用在任何文献中。